The pattern of neural crest advance in the cecum and colon
The pattern of neural crest advance in the cecum and colon
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DOI：
10.1016/j.ydbio.2005.08.040
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发表时间：
2005-11-01
影响因子：
2.7
通讯作者：
Epstein, ML
中科院分区：
文献类型：
--
作者：
Druckenbrod, NR;Epstein, ML
神经嵴细胞离开后脑，进入咽部的肠间充质，并作为细胞束迁移到末端肠以形成肠神经系统。我们通过将Wnt 1-Cre小鼠与Rosa-floxed-YFP小鼠交配产生含有荧光肠神经嵴衍生细胞（ENCC）的胚胎，并使用延时荧光显微镜研究小鼠胚胎完整肠道中的ENCC行为。相对于整个自动机，我们已经发现，在盲肠和近端结肠的ENCC的行为独特。ENCC通过回肠或尾结肠向尾部迁移，逐渐推进链群，显示出很大程度上不可预测的局部轨迹。然而，在盲肠，推进ENCC暂停约12小时，然后显示一个不变的模式迁移到盲肠和近端结肠的不同区域。此外，虽然大多数迁移通过肠道其他区域的ENCC保持相互连接为股; ENCC最初从主要群体迁移通过盲肠和近端结肠片段，并作为分离的单细胞前进。这些细胞聚集成与主网络隔离的组。并最终延伸链本身以在结肠中段重建网络。当ENCC的前进网络到达末端肠时，骶嵴细胞延伸，并与迷走嵴相交以桥接两者之间的小空间。我们发现了ENCC数量，相互作用和迁移行为之间的关系，通过利用内源性孤立的链，并通过沿着ENCC波前切割。根据细胞的数量，ENCC聚集，增殖和延伸链以推进波前。我们的研究结果表明，ENCC之间的相互作用对于调节其发展所需的行为是重要的。(c)2005年爱思唯尔公司All rights reserved.
Neural crest cells leave the hindbrain, enter the gut mesenchyme at the pharynx, and migrate as strands of cells to the terminal bowel to form the enteric nervous system. We generated embryos containing fluorescent enteric neural crest-derived cells (ENCCs) by mating Wnt1-Cre mice with Rosa-floxed-YFP mice and investigated ENCC behavior in the intact gut of mouse embryos using time-lapse fluorescent microscopy. With respect to the entire aut, we have found that ENCCs in the cecum and proximal colon behave uniquely. ENCCs migrating caudally through either the ileum, or caudal colon, are gradually advancing populations of strands displaying largely unpredictable local trajectories. However, in the cecum, advancing ENCCs pause for approximately 12 h, and then display an invariable pattern of migration to distinct regions of the cecum and proximal colon. In addition, while most ENCCs migrating through other regions of the gut remain interconnected as strands; ENCCs initially migrating through the cecum and proximal colon fragment from the main population and advance as isolated single cells. These cells aggregate into groups isolated from the main network. and eventually extend strands themselves to reestablish a network in the mid-colon. As the advancing network of ENCCs reaches the terminal bowel, strands of sacral crest cells extend, and intersect with vagal crest to bridge the small space between. We found a relationship between ENCC number, interaction, and migratory behavior by utilizing endogenously isolated strands and by making cuts along the ENCC wavefront. Depending on the number of cells, the ENCCs aggregated, proliferated, and extended strands to advance the wavefront. Our results show that interactions between ENCCs are important for regulating behaviors necessary for their advancement. (c) 2005 Elsevier Inc. All rights reserved.