Cellular localization of cyclooxygenase-1 and cyclooxygenase-2 in the normal mouse, rat, and human retina
Cellular localization of cyclooxygenase-1 and cyclooxygenase-2 in the normal mouse, rat, and human retina
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DOI：
10.1002/cne.10400
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发表时间：
2002-10-28
影响因子：
2.5
通讯作者：
Neufeld, AH
中科院分区：
文献类型：
--
作者：
Ju, WK;Neufeld, AH
前列腺素由环氧合酶（考克斯）合成，在中枢神经系统中具有调节自主神经反应、传递疼痛、产生发热、控制睡眠-觉醒周期、突触信号传导以及神经元和胶质细胞之间的相互作用等多种神经生理功能。尽管在眼前节组织中已经广泛地研究了异甘草素，但是关于异甘草素在神经视网膜中的作用知之甚少。通过免疫组织化学方法，我们比较了考克斯-1和考克斯-2在正常小鼠、大鼠和人视网膜中的细胞表达和定位。在正常小鼠视网膜中，考克斯-1免疫反应性存在于感光细胞、水平细胞、小胶质细胞、视网膜神经节细胞和移位的无长突细胞的外节中。在正常大鼠视网膜中，考克斯-1免疫反应性存在于小胶质细胞、视网膜神经节细胞和移位的无长突细胞中。在正常人视网膜中，考克斯-1免疫反应性存在于小胶质细胞、星形胶质细胞、视网膜神经节细胞和移位的无长突细胞中。在正常小鼠和大鼠视网膜中，考克斯-2免疫反应性存在于外丛状层的突起和某些无长突细胞和视网膜神经节细胞中。在正常人视网膜中，考克斯-2免疫反应性仅存在于外丛状层的突起中。这些结果表明，由考克斯-1或考克斯-2合成的洋地黄素可能有助于视网膜的正常生理和稳态功能。(C)2002 Wiley-Liss，Inc.
Prostaglandins, synthesized by cyclooxygenase (COX), regulate diverse neurophysiological actions such as regulation of autonomic responses, transmission of pain, generation of fever, control of sleep-wake cycle, synaptic signaling, and cross-talk between neurons and glia in the central nervous system. Although prostaglandins have been widely studied in the anterior segment tissues of the eye, relatively little is known about prostaglandins in the neural retina. By using immunohistochemistry, we have compared the cellular expression and localization of COX-1 and COX-2 in the normal mouse, rat, and human retina. In the normal mouse retina, COX-1 immunoreactivity is present in the outer segments of photoreceptor cells, horizontal cells, microglia, retinal ganglion cells, and displaced amacrine cells. In the normal rat retina, COX-1 immunoreactivity is present in microglia, retinal ganglion cells, and displaced amacrine cells. In the normal human retina, COX-1 immunoreactivity is present in microglia, astrocytes, retinal ganglion cells, and displaced amacrine cells. In the normal mouse and rat retina, COX-2 immunoreactivity is present in processes of the outer plexiform layer and in certain amacrine cells and retinal ganglion cells. In the normal human retina, COX-2 immunoreactivity is only present in processes of the outer plexiform layer. These results suggest that prostaglandins, synthesized by COX-1 or COX-2, may contribute to normal physiological and homeostatic functions in the retina. (C) 2002 Wiley-Liss, Inc.