Stratified scaffold design for engineering composite tissues.
Stratified scaffold design for engineering composite tissues.
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DOI：
10.1016/j.ymeth.2015.03.029
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发表时间：
2015-08
期刊：
影响因子：
4.8
通讯作者：
Christopher Z Mosher;J. Spalazzi;Helen H. Lu
中科院分区：
文献类型：
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作者：
Christopher Z Mosher;J. Spalazzi;Helen H. Lu
骨科软组织修复的一个重要挑战是自体或同种异体移植物与骨的生物固定，这种移植物与宿主骨之间缺乏功能整合，限制了前交叉韧带和其他常见的基于软组织的重建移植物的临床成功。目前的手术重建不能恢复ACL与股骨或胫骨之间的天然纤维软骨附着，从而最大限度地减少应力集中，促进软硬组织之间的负荷转移，损害了这些移植物的长期临床功能。为了实现整合，模拟前交叉韧带界面(韧带-纤维软骨-骨)多个组织区域的分层支架设计代表了一种有希望的复合组织形成策略。此外，通过支架系统内的共培养或三培养获得的独特的细胞组织和特定于相的基质异质性可以促进仿生多组织再生。在这里，我们描述了用于韧带-骨整合的三相支架的制备方法，以及成纤维细胞、软骨细胞和成骨细胞在分层支架上的三种培养方法，以形成韧带、纤维软骨和骨的结构连续和成分不同的区域。三相支架的主要优点是通过分层设计重现了跨自然界面的多组织组织。此外，除了易于制造外，每个支架相在聚合物组成上相似，因此可以通过烧结连接在一起，从而实现每个区域的无缝整合，并避免支架层之间的分层。
A significant challenge to orthopaedic soft tissue repair is the biological fixation of autologous or allogeneic grafts with bone, whereby the lack of functional integration between such grafts and host bone has limited the clinical success of anterior cruciate ligament (ACL) and other common soft tissue-based reconstructive grafts. The inability of current surgical reconstruction to restore the native fibrocartilaginous insertion between the ACL and the femur or tibia, which minimizes stress concentration and facilitates load transfer between the soft and hard tissues, compromises the long-term clinical functionality of these grafts. To enable integration, a stratified scaffold design that mimics the multiple tissue regions of the ACL interface (ligament–fibrocartilage–bone) represents a promising strategy for composite tissue formation. Moreover, distinct cellular organization and phase-specific matrix heterogeneity achieved through co- or tri-culture within the scaffold system can promote biomimetic multi-tissue regeneration. Here, we describe the methods for fabricating a tri-phasic scaffold intended for ligament–bone integration, as well as the tri-culture of fibroblasts, chondrocytes, and osteoblasts on the stratified scaffold for the formation of structurally contiguous and compositionally distinct regions of ligament, fibrocartilage and bone. The primary advantage of the tri-phasic scaffold is the recapitulation of the multi-tissue organization across the native interface through the layered design. Moreover, in addition to ease of fabrication, each scaffold phase is similar in polymer composition and therefore can be joined together by sintering, enabling the seamless integration of each region and avoiding delamination between scaffold layers.