Bombyx mori nucleopolyhedrovirus F-like protein Bm14 is a factor for viral-induced cytopathic effects via regulating oxidative phosphorylation and cellular ROS levels
Bombyx mori nucleopolyhedrovirus F-like protein Bm14 is a factor for viral-induced cytopathic effects via regulating oxidative phosphorylation and cellular ROS levels
复制标题
家蚕核多角体病毒 F 样蛋白 Bm14 是通过调节氧化磷酸化和细胞 ROS 水平引起病毒诱导的细胞病变效应的因子
DOI：
10.1016/j.virol.2020.10.001
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发表时间：
2021-01-02
期刊：
影响因子：
3.7
通讯作者：
Wu, Xiaofeng
中科院分区：
文献类型：
--
作者：
Kong, Xiangshuo;Xu, Weifan;Wu, Xiaofeng
家蚕核多角体病毒（Bombyx mori polypolyhedrovirus, BmNPV）是家蚕的高致病性病毒，感染后可引起严重的细胞病变（cytop病变）。然而，病毒蛋白在病毒诱导的CPEs中的作用尚不清楚。在这里，我们发现BmNPV感染诱导了严重的cpe，包括滴度依赖性细胞漂浮和细胞表面形态的变化。进一步的研究发现，病毒包膜蛋白f样蛋白（Bm14）参与了诱导细胞毒性和粘附BmN细胞脱离的过程，其破坏显著削弱了病毒感染介导的cpe。有趣的是，转录组学分析发现Bm14缺失与细胞氧化磷酸化途径的激活密切相关，这与线粒体膜电位（MMP）水平和ATP浓度升高以及ROS水平降低一致。总之，我们的研究结果首次揭示了Bm14通过抑制细胞氧化磷酸化水平和上调ROS水平来加速病毒诱导的细胞致病性的新作用。
Bombyx mori nucleopolyhedrovirus (BmNPV) is highly pathogenic to Bombyx mori, silkworm, which causes serious cytopathic effects (CPEs) during infection. However, the role of viral protein in the virus-induced CPEs remains unclear. Here, we discovered that BmNPV infection induced severe CPEs including titer-dependent cell floating and changes in cellular surface morphology. Further explorations revealed the involvement of F-like protein (Bm14), a viral envelope protein, in inducing cytotoxicity and detachment of adherent BmN cells, and its disruption significantly impaired the virus infection-mediated CPEs. Intriguingly, transcriptomic analysis identified the tight association of Bm14 deletion with the activation of cellular oxidative phosphorylation pathway, consistent with the elevated mitochondrial membrane potential (MMP) levels and ATP concentrations as well as reduced ROS levels. Collectively, our results characterized for the first time the novel role of Bm14 in accelerating viral-induced cytopathogenicity via suppressing the cellular oxidative phosphorylation levels and upregulating the ROS levels.