Anti-α-fodrin inhibits secretion from permeabilized chromaffin cells
Anti-α-fodrin inhibits secretion from permeabilized chromaffin cells
复制标题
抗α-胞质蛋白抑制透化嗜铬细胞的分泌
作者：
D. Perrin;O. Langley;D. Aunis
嗜铬细胞通过胞吐1 -5释放含儿茶酚胺和肽的颗粒，其机制涉及分泌颗粒向细胞膜的移动、它们与细胞膜的并置以及颗粒膜与质膜的融合。α-胞衬蛋白是膜相关脑血影蛋白的两个亚基之一，是一种肌动蛋白结合蛋白，存在于嗜铬细胞的外周，可能参与分泌6。因为培养的嗜铬细胞可以用去污剂透化7 -9，产生足够大的孔以允许免疫球蛋白分子进入，我们使用透化的细胞来测试特异性抗体对分泌机制的影响。用多克隆免疫亲和纯化的单特异性抗α-胞衬蛋白抗体或其Fab片段孵育透化细胞，不改变基础释放，但特异性抑制Ca 2+诱导的胞吐儿茶酚胺释放。我们的观察表明，胞衬蛋白和细胞骨架参与的释放机制。
Chromaffin cells release catecholamine- and peptide-containing granules by exocytosis1–5, by a mechanism involving movement of secretory granules towards the cell membrane, their apposition to it and the fusion of the granule membrane with the plasma membrane. One of the two subunits of membrane-associated brain spectrin, α-fodrin is an actin-binding protein which is found at the periphery of chromaffin cells and may be involved in secretion6. Because cultured chromaffin cells can be permeabilized with detergents7–9, giving pores large enough to permit the entry of immunoglobulin molecules, we used permeabilized cells to test the effect of specific antibodies on secretory mechanisms. Incubation of permeabilized cells with polyclonal immunoaffinity-purified monospecific anti-α-fodrin antibody or its Fab fragments did not modify basal release but did specifically inhibit Ca2+-induced catecholamine release by exocytosis. Our observations indicate that fodrin and the cytoskeleton participate in the release mechanism.
洋地黄皂苷通透的肾上腺髓质嗜铬细胞引起钙分泌。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Wilson,SP;Kirshner,N
通讯作者：
Kirshner,N
DOI：
10.1016/s0021-9258(18)32526-2
发表时间：
1983-04
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
L. Dunn;R. Holz
通讯作者：
L. Dunn;R. Holz
脑血影蛋白。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Davis,J;Bennett,V
通讯作者：
Bennett,V