HORMONE-SENSITIVE LIPASE AND MONOACYLGLYCEROL LIPASE ARE BOTH REQUIRED FOR COMPLETE DEGRADATION OF ADIPOCYTE TRIACYLGLYCEROL
HORMONE-SENSITIVE LIPASE AND MONOACYLGLYCEROL LIPASE ARE BOTH REQUIRED FOR COMPLETE DEGRADATION OF ADIPOCYTE TRIACYLGLYCEROL
复制标题
DOI：
10.1016/0005-2760(86)90286-9
复制
发表时间：
1986-04-15
期刊：
影响因子：
--
通讯作者：
BELFRAGE, P
中科院分区：
文献类型：
--
作者：
FREDRIKSON, G;TORNQVIST, H;BELFRAGE, P
研究了单酰基甘油脂酶和脂肪敏感脂肪酶在脂肪组织三酰基甘油连续水解中的作用。含有与完整组织中大致相同比例的两种脂肪酶的脂肪组织制剂将乳化的三油酰甘油或二油酰甘油水解为脂肪酸和甘油，几乎没有二油酰甘油或单油酰甘油的积累。通过免疫沉淀选择性去除单酰基甘油脂酶显着降低甘油释放。根据该酶的位置特异性，分离的脂肪酶敏感性脂肪酶水解酰基甘油，具有单酰基甘油的显著积累（Fredrikson，G.和Belfrage，P.（1983）J.Biol.Chem.258，14253-14256）。增加量的分离的单酰基甘油脂肪酶的添加导致甘油释放的相应增加，由于形成的单酰基甘油的水解。当两种脂肪酶的相对比例与完整组织中的相似时，反应进行至完成。这些研究结果表明，脂肪酶敏感的脂肪酶催化的三酰甘油的水解在脂肪组织脂解的限速步骤，和所得的二酰甘油，而单酰基甘油脂肪酶的行动是需要在最终水解的2-monoacylglycerols生产。
The respective roles of monoacylglycerol lipase and hormone-sensitive lipase in the sequential hydrolysis of adipose tissue triacylglycerols have been examined. An adipose tissue preparation, containing both lipases in approximately the same proportion as in the intact tissue, hydrolyzed emulsified tri- or dioleoylglycerol to fatty acids and glycerol, with little accumulation of di- or monooleoylglycerol. Selective removal of the monoacylglycerol lipase by immunoprecipitation markedly reduced the glycerol release. Isolated hormone-sensitive lipase hydrolyzed acylglycerols with a marked accumulation of monoacylglycerol in accordance with the positional specificity of this enzyme (Fredrikson, G. and Belfrage, P. (1983) J. Biol. Chem. 258, 14253-14256). Addition of increasing amounts of isolated monoacylglycerol lipase led to a corresponding increase in glycerol release, due to hydrolysis of the monoacylglycerols formed. The reaction proceeded to completion when the relative proportion of the two lipases was similar to that in the intact tissue. These findings indicate that hormone-sensitive lipase catalyzes the hydrolysis of triacylglycerol in the rate-limiting step of adipose tissues lipolysis, and of the resulting diacylglycerol, whereas the action of monoacylglycerol lipase is required in the final hydrolysis of the 2-monoacylglycerols produced.