Natural selection for polymorphism in the disease resistance gene Rps2 of Arabidopsis thaliana.
Natural selection for polymorphism in the disease resistance gene Rps2 of Arabidopsis thaliana.
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发表时间：
2003-02
期刊：
影响因子：
3.3
通讯作者：
R. Mauricio;E. Stahl;T. Korves;Dacheng Tian;M. Kreitman;J. Bergelson
中科院分区：
文献类型：
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作者：
R. Mauricio;E. Stahl;T. Korves;Dacheng Tian;M. Kreitman;J. Bergelson
病原体抗性是一种重要的生态表型，在分子遗传水平上的认识越来越深入。在这篇文章中，我们研究了在世界范围内的样本27加入拟南芥avrRpt 2依赖性的电阻和Rps 2基因座DNA序列变异的水平。Rps 2序列从一个不同的生态型的根简约树，包括一个深分叉分离的主要抗性和易感性的等位基因分支。我们发现的证据，保持这些等位基因的选择，并确定N-末端部分的亮氨酸丰富的重复区域作为一个可能的目标选择。在两个主要分支中发现了其他蛋白质变体，并且与对病原体假单胞菌的无毒基因avrRpt 2的抗性的生态型之间的可测量差异相关。在其他的抗性基因上也观察到了长寿命的多态性。thaliana; Rps 2数据表明，抗性基因中表型变异的长期维持可能是一种普遍现象，并且与多样化选择一致，多样化选择与维持变异的选择一致。
Pathogen resistance is an ecologically important phenotype increasingly well understood at the molecular genetic level. In this article, we examine levels of avrRpt2-dependent resistance and Rps2 locus DNA sequence variability in a worldwide sample of 27 accessions of Arabidopsis thaliana. The rooted parsimony tree of Rps2 sequences drawn from a diverse set of ecotypes includes a deep bifurcation separating major resistance and susceptibility clades of alleles. We find evidence for selection maintaining these alleles and identify the N-terminal part of the leucine-rich repeat region as a probable target of selection. Additional protein variants are found within the two major clades and correlate well with measurable differences among ecotypes in resistance to the avirulence gene avrRpt2 of the pathogen Pseudomonas syringae. Long-lived polymorphisms have been observed for other resistance genes of A. thaliana; the Rps2 data suggest that the long-term maintenance of phenotypic variation in resistance genes may be a general phenomenon and are consistent with diversifying selection acting in concert with selection to maintain variation.