Calcium-Differentiated Cellular Internalization of Allosteric Framework Nucleic Acids for Targeted Payload Delivery.
Calcium-Differentiated Cellular Internalization of Allosteric Framework Nucleic Acids for Targeted Payload Delivery.
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DOI：
10.1021/acs.analchem.2c01434
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发表时间：
2022-06
影响因子：
7.4
通讯作者：
Jiao Zheng;Qiwei Wang;Lin Shi;Lili Shi;Tao Li
中科院分区：
文献类型：
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作者：
Jiao Zheng;Qiwei Wang;Lin Shi;Lili Shi;Tao Li
靶向递送系统在癌症治疗中已广泛显示出有前途的应用，其中许多靶向递送系统通过响应癌细胞微环境而巧妙地发挥作用。在这里，我们首次报告了2D/3D框架核酸（FNA）的Ca 2+分化细胞内化，从而能够使用变构FNA纳米载体设计概念上新的靶向递送系统。FNA载体通过响应环境K+的G-四链体在癌细胞表面上进行2D至3D转化，从而允许其细胞进入被Ca 2+更有效地促进。这种设计使FNA载体能够靶向癌细胞并选择性地递送含有反义链的货物用于活细胞mRNA成像。它将为靶向药物输送开辟新的途径，并在精确的疾病治疗中找到广泛的应用。
Target delivery systems have extensively shown promising applications in cancer therapy, and many of them function smartly by responding to the cancer cell microenvironment. Here, we for the first time report Ca2+-differentiated cellular internalization of 2D/3D framework nucleic acids (FNAs), enabling the engineering of a conceptually new target delivery system using an allosteric FNA nanovehicle. The FNA vehicle is subject to a 2D-to-3D transformation on the cancer cell surface via G-quadruplexes responding to environmental K+ and thereby allows its cell entry to be more efficiently promoted by Ca2+. This design enables the FNA vehicle to target cancer cells and selectively deliver an antisense strand-containing cargo for live-cell mRNA imaging. It would open new avenues toward targeted drug delivery and find extensive applications in precise disease treatment.