BINDING OF GERM-CELLS TO MUTANT SL(D) SERTOLI CELLS IS DEFECTIVE AND IS RESCUED BY EXPRESSION OF THE TRANSMEMBRANE FORM OF THE C-KIT LIGAND
BINDING OF GERM-CELLS TO MUTANT SL(D) SERTOLI CELLS IS DEFECTIVE AND IS RESCUED BY EXPRESSION OF THE TRANSMEMBRANE FORM OF THE C-KIT LIGAND
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DOI：
10.1006/dbio.1993.1122
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发表时间：
1993-05-01
影响因子：
2.7
通讯作者：
SORRENTINO, V
中科院分区：
文献类型：
--
作者：
MARZIALI, G;LAZZARO, D;SORRENTINO, V
编码c-kit配体（KL）的stel（S1）基因座的突变与小鼠生殖细胞发育受损相关。存在两种形式的KL：一种更稳定地与质膜结合，另一种更容易作为可溶性蛋白释放。我们在这里报告的表达的两种mRNA编码的两种不同形式的ofKL是发育调节小鼠睾丸。在出生时，这两种mRNA以相等的比例表达。出生后6天开始，与生殖细胞分化的开始平行，编码膜相关形式KL的mRNA变得更加丰富。生殖细胞，特别是精原细胞，表达c-kit，因此膜结合KL可以介导支持细胞和生殖细胞之间的粘附。我们发现，事实上，从Sl/Sld突变小鼠，不表达的KL的膜相关形式的mRNA的支持细胞，是不能结合生殖细胞。导入表达KL跨膜形式而非可溶形式的质粒可恢复S1/Sld突变小鼠的支持细胞结合生殖细胞的能力。这些数据表明，KLin支持细胞的膜锚定形式的优先表达可能在介导表达c-kit的生殖细胞与支持细胞的粘附中起作用。
Mutations in thesteel(Sl) locus, encoding the c-kitligand (KL), are associated with impaired germ cell development in mice. Two forms ofKLexist: one more steadily associated with the plasma membrane and one more easily released as a soluble protein. We report here that the expression of the two mRNAs coding for the two different form ofKLis developmentally regulated in mouse testis. At birth the two mRNAs are expressed at an equal ratio. Starting after 6 days of life, and in parallel to initiation of germ cell differentiation, the mRNA encoding the membrane-associated form ofKLbecomes more abundant. Germ cells, and especially spermatogonia, express c-kit;thus membrane-boundKLcould mediate adhesion between Sertoli cells and germ cells. We find, in fact, that Sertoli cells from Sl/Sldmutant mice, which do not express the mRNA for the membrane-associated form ofKL,are unable to bind germ cells. Introduction of a plasmid expressing the transmembrane form, but not the soluble form, ofKLrestores the ability of Sertoli cells from Sl/Sldmutant mice to bind germ cells. These data suggest that preferential expression of the membrane-anchored form ofKLin Sertoli cells may have a role in mediating adhesion of c-kit-expressing germ cells to Sertoli cells.