Isolation of genes active during hormone-induced morphogenesis in Drosophila imaginal discs.
Isolation of genes active during hormone-induced morphogenesis in Drosophila imaginal discs.
复制标题
果蝇成虫盘激素诱导形态发生过程中活跃基因的分离。
DOI：
10.1016/s0021-9258(19)57253-2
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
J. Fristrom
中科院分区：
文献类型：
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作者：
J. Natzle;A. Hammonds;J. Fristrom
体外培养的大量分离的果蝇影像盘在昆虫类固醇激素20-羟基蜕皮激素的作用下发生形态发生（外翻）。从外翻椎间盘分离的mRNA的体外翻译显示至少有5个新的mRNA转录物的积累，这些转录物仅存在于膜结合的多体RNA中，可能编码影像椎间盘膜或分泌蛋白。采用改良的差动杂交筛选技术，分离了6个不同的激素诱导膜蛋白基因序列。这些基因构成了一组新的20-羟基蜕皮激素应答位点，可能编码影像椎间盘形态发生所需的基因产物。
Mass-isolated Drosophila imaginal discs cultured in vitro undergo morphogenesis (evagination) in response to the insect steroid hormone 20-hydroxyecdysone. In vitro translation of mRNA isolated from evaginating discs shows accumulation of at least five new mRNA transcripts that are present only in membrane-bound polysomal RNA and presumably encode imaginal disc membrane or secreted proteins. Using a modified differential hybridization screen employing a competition step, six different hormone-inducible membrane protein gene sequences were isolated. These genes constitute a new set of 20-hydroxyecdysone responsive loci that may encode gene products specifically required for imaginal disc morphogenesis.