HETEROGENEITY OF BOVINE LACTOTRANSFERRIN GLYCANS - CHARACTERIZATION OF ALPHA-D-GALP-(1-]3)-BETA-D-GAL AND ALPHA-NEUAC-(2-]6)-BETA-D-GALPNAC-(1-]4)-BETA-D-GLCNAC-SUBSTITUTED N-LINKED GLYCANS
HETEROGENEITY OF BOVINE LACTOTRANSFERRIN GLYCANS - CHARACTERIZATION OF ALPHA-D-GALP-(1-]3)-BETA-D-GAL AND ALPHA-NEUAC-(2-]6)-BETA-D-GALPNAC-(1-]4)-BETA-D-GLCNAC-SUBSTITUTED N-LINKED GLYCANS
复制标题
DOI：
10.1016/0008-6215(92)85013-p
复制
发表时间：
1992-12-15
影响因子：
3.1
通讯作者：
SPIK, G
中科院分区：
文献类型：
--
作者：
CODDEVILLE, B;STRECKER, G;SPIK, G
从成熟牛奶中分离出的乳转铁蛋白含有n -糖苷连接的聚糖，这些聚糖具有n -乙酰神经氨酸、半乳糖、甘露糖、焦糖、n -乙酰氨基葡萄糖和n -乙酰半乳糖胺。将经Pronase酶切得到的糖肽通过刀豆蛋白A- sepharose亲和层析分离成轻度保留(A)、保留(B)和强烈保留(C)三个部分。采用甲醇解、甲基化分析、快速原子轰击-质谱法和核磁共振氢谱法测定了三个组分的聚糖结构。在A和b部分中，不含GalNAc的天线结构以部分唾液化和部分（1—bbb6）- α - l -聚焦结构的形式存在。在α - d - man -(1 -> 3)天线上对α - d - galpnac -(1—> 4)- β - d - glcnac和α - neuac -(2—> 6)- β - d - galpnac -(1—> 4)- β - d - glcnac进行了表征。在α - d - man -（1—> 3）天线上对α - d - galpnac -(1—> 4)- β - d - glcnac进行了酶切。这些结果表明，牛乳转铁蛋白聚糖的结构比先前表征的转铁蛋白聚糖更具异质性。
Lactotransferrin isolated from a pool of mature bovine milk has been shown to contain N-glycosidically-linked glycans possessing N-acetylneuraminic acid, galactose, mannose, fucose, N-acetylglucosamine, and N-acetylgalactosamine. The glycopeptides obtained by Pronase digestion were fractionated by concanavalin A-Sepharose affinity chromatography into three fractions: slightly retained (A), retained (B), and strongly retained (C). The structure of the glycans of the three fractions has been determined by application of methanolysis, methylation analysis, fast atom bombardment-mass spectrometry, and H-1 NMR spectroscopy. Diantennary structures without GalNAc were present as partially sialylated and partially (1 --> 6)-alpha-L-fucosylated structures in Fractions A and B. Sequences containing alpha-D-Galp-(1 --> 3)-beta-D-Gal on the alpha-D-Man-(1 --> 6) antenna, and beta-D-GalpNAc-(1 --> 4)-beta-D-GlcNAc and alpha-NeuAc-(2 --> 6)-beta-D-GalpNAc-(1 --> 4)-beta-D-GlcNAc on the alpha-D-Man-(1 --> 3) antenna were characterized in the oligosaccharide-alditols obtained by reductive cleavage of Fraction B. A series of Man4-9-GlcNAc structures were identified in Fraction C after endo-N-acetyl-beta-D-glucosaminidase digestion. These results show that the structures of bovine lactotransferrin glycans are more heterogeneous than those of previously characterized transferrin glycans.