Transcription factor PRO1 targets genes encoding conserved components of fungal developmental signaling pathways
Transcription factor PRO1 targets genes encoding conserved components of fungal developmental signaling pathways
复制标题
DOI：
10.1111/mmi.13491
复制
发表时间：
2016-08
影响因子：
3.6
通讯作者：
Eva Katharina Steffens;Kordula Becker;Sabine Krevet;I. Teichert;U. Kück
中科院分区：
文献类型：
--
作者：
Eva Katharina Steffens;Kordula Becker;Sabine Krevet;I. Teichert;U. Kück
丝状真菌大孢子实体是研究子实体形成过程中多细胞发育的模式系统。在此之前，我们证明了性生活周期中的这一主要过程是由锌(II)2Cys6锌簇转录因子Pro1控制的。在这里，我们进一步研究了由Pro1控制的全基因组调控网络，采用染色质免疫沉淀结合下一代测序(CHIP-SEQ)来确定Pro1的结合位点。我们确定了在编码不同信号通路组件的基因的启动子区域中出现的几个靶区。此外，我们在体外发现了一个与Pro1特异结合的保守的DNA结合基序。此外，Pro1在体内控制ESDC靶基因启动子控制下的DsRed报告基因的表达。我们的CHIP-SEQ数据表明，Pro1还控制着以前被证明参与调控控制细胞壁完整性、NADPH氧化酶和信息素信号的途径的靶基因。我们的数据表明，Pro1作为信号组件的基因的主调节器，组成了控制子实体形成的发育级联。
The filamentous fungus Sordaria macrospora is a model system to study multicellular development during fruiting body formation. Previously, we demonstrated that this major process in the sexual life cycle is controlled by the Zn(II)2Cys6 zinc cluster transcription factor PRO1. Here, we further investigated the genome‐wide regulatory network controlled by PRO1 by employing chromatin immunoprecipitation combined with next‐generation sequencing (ChIP‐seq) to identify binding sites for PRO1. We identified several target regions that occur in the promoter regions of genes encoding components of diverse signaling pathways. Furthermore, we identified a conserved DNA‐binding motif that is bound specifically by PRO1 in vitro. In addition, PRO1 controls in vivo the expression of a DsRed reporter gene under the control of the esdC target gene promoter. Our ChIP‐seq data suggest that PRO1 also controls target genes previously shown to be involved in regulating the pathways controlling cell wall integrity, NADPH oxidase and pheromone signaling. Our data point to PRO1 acting as a master regulator of genes for signaling components that comprise a developmental cascade controlling fruiting body formation.