Tween-20 transiently changes the surface morphology of PK-15 cells and improves PCV2 infection.
Tween-20 transiently changes the surface morphology of PK-15 cells and improves PCV2 infection.
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DOI：
10.1186/s12917-018-1457-5
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发表时间：
2018-04-24
影响因子：
2.6
通讯作者：
Hou J
中科院分区：
文献类型：
--
作者：
Hua T;Zhang X;Tang B;Chang C;Liu G;Feng L;Yu Y;Zhang D;Hou J
低浓度的非离子表面活性剂可以改变细胞膜的物理性质，从而反过来增加药物渗透性。猪圆环病毒2（PCV 2）是一种生长极其缓慢的病毒，PK-15细胞的PCV 2感染产生非常低的病毒滴度。本研究调查了各种非离子表面活性剂，即Tween-20、Tween-28、Tween-40、Tween-80、Brij-30、Brij-35、NP-40和Triton X-100对PK-15细胞中PCV 2感染和产量的影响。与用Tween-28、Tween-40、Brij-30、Brij-35、NP-40和Triton X-100处理的细胞相比，在用Tween-20处理的细胞中观察到显著增加的PCV 2感染（p < 0.01）。此外，用0.03%吐温-20孵育24小时已显示诱导显著的细胞形态学变化（细胞膜轻度肿胀，微绒毛减少），caspase-3活性增加，细胞活力降低;所有这些变化在吐温-20从板上洗出后恢复正常。我们的数据表明，吐温-20瞬时改变PK-15细胞的表面形态，并改善PCV 2感染。本研究的结果可用于PCV 2疫苗的开发。
Low concentrations of nonionic surfactants can change the physical properties of cell membranes, and thus and in turn increase drug permeability. Porcine circovirus 2 (PCV2) is an extremely slow-growing virus, and PCV2 infection of PK-15 cells yields very low viral titers. The present study investigates the effect of various nonionic surfactants, namely, Tween-20, Tween-28, Tween-40, Tween-80, Brij-30, Brij-35, NP-40, and Triton X-100 on PCV2 infection and yield in PK-15 cells. Significantly increased PCV2 infection was observed in cells treated with Tween-20 compared to those treated with Tween-28, Tween-40, Brij-30, Brij-35, NP-40, and Triton X-100 (p < 0.01). Furthermore, 24 h incubation with 0.03% Tween-20 has shown to induce significant cellular morphologic changes (cell membrane underwent slight intumescence and bulged into a balloon, and the number of microvilli decreased), as well as to increase caspase-3 activity and to decrease cell viability in PCV2-infected PK-15 cells cmpared to control group; all these changes were restored to normal after Tween-20 has been washed out from the plate. Our data demonstrate that Tween-20 transiently changes the surface morphology of PK-15 cells and improves PCV2 infection. The findings of the present study may be utilized in the development of a PCV2 vaccine.
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影响因子：
5.4
作者：
Misinzo, Gerald;Delputte, Peter L.;Nauwynck, Hans J.
通讯作者：
Nauwynck, Hans J.
影响因子：
3.8
作者：
SARPOTDAR, PP;ZATZ, JL
通讯作者：
ZATZ, JL
影响因子：
5
作者：
Misinzo, G.;Delputte, P. L.;Nauwynck, H. J.
通讯作者：
Nauwynck, H. J.
影响因子：
2.3
作者：
Meerts, P;Misinzo, G;Nauwynck, HJ
通讯作者：
Nauwynck, HJ
影响因子：
3.4
作者：
MARSH, RJ;MAURICE, DM
通讯作者：
MAURICE, DM