Transcriptional and posttranscriptional regulation of exogenous human beta interferon gene in simian cells defective in interferon synthesis
Transcriptional and posttranscriptional regulation of exogenous human beta interferon gene in simian cells defective in interferon synthesis
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DOI：
10.1128/mcb.6.6.2279-2283.1986
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发表时间：
1986-06
影响因子：
5.3
通讯作者：
J. Mosca;P. Pitha
中科院分区：
文献类型：
--
作者：
J. Mosca;P. Pitha
我们确定，在Vero细胞中β干扰素诱导的缺陷是由于缺乏猿猴β干扰素（IFN-β）基因。然而，人IFN-β基因或其中人IFN-β启动子调控区指导氯霉素乙酰转移酶基因（pIFN-CAT）表达的杂合基因可以在转染的Vero细胞中被诱导，并且这些细胞也在转录后调节IFN-β mRNA（但不是pIFN-CAT mRNA）。这些结果表明，人IFN-β基因的不稳定性是由IFN-β mRNA的编码区或3 ′-末端区编码的，并且人IFN-β基因在Vero和人细胞中以相同的方式受到调节。
We determined that the defect in beta interferon induction in Vero cells is due to the absence of the simian beta interferon (IFN-beta) gene. Nevertheless, the human IFN-beta gene or a hybrid gene, in which the human IFN-beta promoter-regulatory region directs expression of the chloramphenicol acetyltransferase gene (pIFN-CAT), could be induced in transfected Vero cells, and these cells also regulated IFN-beta mRNA (but not pIFN-CAT mRNA) posttranscriptionally. These results indicate that the instability in the human IFN-beta gene is coded for by the coding or 3'-end region of IFN-beta mRNA and that the human IFN-beta gene is regulated in Vero and human cells in an identical manner.