Organ printing: promises and challenges
Organ printing: promises and challenges
复制标题
DOI：
10.2217/17460751.3.1.93
复制
发表时间：
2008-01-01
影响因子：
2.7
通讯作者：
Markwald, Roger R.
中科院分区：
文献类型：
--
作者：
Mironov, Vladimir;Kasyanov, Vladimir;Markwald, Roger R.
器官打印或快速成型的生物医学应用，也被定义为添加剂逐层生物制造，是一种新兴的转化技术，有可能超越传统的基于固体支架的组织工程。器官打印具有一定的优势：它是一种自动化方法，为组织工程产品的可扩展可重复大规模生产提供了途径;它允许几种细胞类型的精确同时3D定位;它能够创建具有高水平细胞密度的组织;它可以解决厚组织结构中的血管化问题;最后，器官打印可以原位完成。器官打印技术的最终目标是制造适合临床植入的3D血管化功能性活体人体器官。器官打印技术的主要实际成果是复杂人体组织和器官的工业可扩展机器人生物制造，用于临床诊断，药物发现和药物毒性的自动化基于组织的体外测定，以及人类疾病的复杂体外模型。本文介绍了器官印刷技术的概念框架和最新发展，概述了主要的技术障碍和挑战，并提出了潜在的未来实际应用。
Organ printing or biomedical application of rapid prototyping, also defined as additive layer-by-layer biomanufacturing, is an emerging transforming technology that has potential for surpassing traditional solid scaffold-based tissue engineering. Organ printing has certain advantages: it is an automated approach that offers a pathway for scalable reproducible mass production of tissue engineered products; it allows a precised simultaneous 3D positioning of several cell types; it enables creation tissue with a high level of cell density; it can solve the problem of vascularization in thick tissue constructs; finally, organ printing can be done in situ. The ultimate goal of organ-printing technology is to fabricate 3D vascularized functional living human organs suitable for clinical implantation. The main practical outcomes of organ-printing technology are industrial scalable robotic biofabrication of complex human tissues and organs, automated tissue-based in vitro assays for clinical diagnostics, drug discovery and drug toxicity, and complex in vitro models of human diseases. This article describes conceptual framework and recent developments in organ-printing technology, outlines main technological barriers and challenges, and presents potential future practical applications.