Biological Effects of Provisional Resin Materials on Human Dental Pulp Stem Cells
Biological Effects of Provisional Resin Materials on Human Dental Pulp Stem Cells
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DOI：
10.2341/16-137-l
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发表时间：
2017-03-01
影响因子：
2.2
通讯作者：
Lee, J-H
中科院分区：
文献类型：
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作者：
Jun, S-K;Mahapatra, C.;Lee, J-H
目的：方法：选择SNAP [SN]、Luxatemp [LT]、Jet [JE]、Revotek LC [RL]和Vipi block [VB] 5种市售临时树脂材料，对原代培养的人牙髓干细胞（hDPSCs）进行细胞毒性和促炎细胞因子表达的研究。将正在聚合或已经凝固的洗脱液在分为三个不同凝固时间（25%凝固、50%凝固和100%凝固）的连续稀释条件下添加到hDPSC中，并用2x浓缩的培养基孵育24小时。通过LDH测定和活细胞和死细胞共聚焦显微镜图像进行细胞毒性试验。用细胞因子抗体芯片检测SN和VB中促炎细胞因子的表达。结果：50%稀释培养液中SN、LT和JE在已定型阶段的细胞毒性均大于30%（p <0.05），而SN和LT在早期或中期的样品中均能检测到细胞毒性。如通过活的和死的图像所观察到的，与来自已经设定的样品的洗脱液相比，来自聚合阶段的洗脱液的SN、JE和LT的细胞毒性更大（p < 0.05）。另一方面，RL和VB没有表现出大于30%的细胞毒性。促炎性细胞因子未检测到12.5%稀释培养物与VB和早期设置阶段SN的洗脱液。结论：从化学活化的临时树脂材料在聚合过程中（SN，LT，和JE）的洗脱液是细胞毒性的hDPSCs，并可能对牙髓组织产生不利影响。
Objectives: This study investigated the in vitro cytotoxicity as well as the proinflammatory cytokine expression of provisional resin materials on primary cultured human dental pulp stem cells (hDPSCs).Methods: Five commercially available provisional resin materials were chosen (SNAP [SN], Luxatemp [LT], Jet [JE], Revotek LC [RL], and Vipi block [VB]). Eluates that were either polymerizing or already set were added to hDPSCs under serially diluted conditions divided into three different setting times (25% set, 50% set, and 100% set) and incubated for 24 hours with 2xconcentrated culture media. Cell cytotoxicity tests were performed by LDH assay and live and dead confocal microscope images. The expression of proinflammatory cytokines in SN and VB was measured using cytokine antibody arrays. Data were analyzed using repeated measures analysis of variance (ANOVA) or ANOVA followed by the Tukey post hoc test at a significance level of p < 0.05.Results: Cytotoxicity greater than 30% was observed in the 50% diluted culture in SN, LT, and JE in the already set stage (p < 0.05), while it was detected in SN and LT in early or intermediate stage samples. The cytotoxicity of SN, JE, and LT was greater with eluates from the polymerizing phase compared to that from already set samples (p < 0.05), as observed by live and dead images. On the other hand, RL and VB did not exhibit cytotoxicity greater than 30%. Proinflammatory cytokines were not detected in 12.5% diluted culture with eluates from VB and early set stage SN.Conclusions: The eluates from chemical-activated provisional resin materials during polymerization (SN, LT, and JE) were cytotoxic to hDPSCs and may adversely affect pulp tissue.