Detection of cariogenic Streptococcus mutans in extirpated heart valve and atheromatous plaque specimens
Detection of cariogenic Streptococcus mutans in extirpated heart valve and atheromatous plaque specimens
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DOI：
10.1128/jcm.00377-06
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发表时间：
2006-09-01
影响因子：
9.4
通讯作者：
Ooshima, Takashi
中科院分区：
文献类型：
--
作者：
Nakano, Kazuhiko;Inaba, Hiroaki;Ooshima, Takashi
口腔细菌在心血管疾病发病机制中的作用一直是许多研究关注的焦点，在心血管疾病的病变中发现了几种牙周病原体，提示口腔微生物与心血管疾病之间的关系。然而，没有关于致龋性病原体，如变形链球菌的参与的信息。应用聚合酶链式反应方法对35例心脏瓣膜和27例动脉粥样硬化斑块临床标本以及32例牙菌斑标本进行口腔链球菌和牙周炎相关细菌的检测。此外，采用广谱聚合酶链式反应和DNA测序分析对样品中的细菌种类进行鉴定。变形链球菌在心脏瓣膜(69%)和动脉粥样斑块(74%)标本中检出较多，而其他细菌种类，包括与牙周炎有关的细菌，检出频率较低。心血管组织中的细菌组成与牙菌斑中的细菌组成明显不同，只有少数种类的心血管区域可能起源于口腔，其中包括变形链球菌。半定量检测结果显示，在心脏瓣膜(40%)和动脉粥样斑块(48%)标本中检测到大量变形链球菌，而在心血管标本中检测到所有其他被测细菌的数量，包括与牙周炎有关的几种细菌。这些结果表明，变形链球菌可能是心血管疾病的病原体。
The involvement of oral bacteria in the pathogenesis of cardiovascular diseases has been the focus of attention in many studies, and several periodontal pathogens have been detected in diseased cardiovascular lesions, suggesting relationships between oral microorganisms and cardiovascular diseases. However, no information is available regarding the involvement of cariogenic pathogens such as Streptococcus mutans. The presence of oral streptococcal species and periodontitis-related bacteria in 35 heart valve and 27 atheromatous plaque clinical specimens, as well as 32 dental plaque specimens from the same subjects, was analyzed using a PCR method. Furthermore, broad-range PCR with DNA sequencing analysis was employed to identify the bacterial species in those samples. Streptococcus mutans was frequently detected in the heart valve (69%) and atheromatous plaque (74%) specimens, while other bacterial species, including those related to periodontitis, were detected with much lower frequencies. The bacterial composition in cardiovascular tissues was found to be markedly distinct from that in dental plaque, with only a limited number of species, including S. mutans, in the cardiovascular regions shown to have possibly originated from the oral cavity. Semiquantitative assay results revealed that S. mutans was detected in significant quantities in the heart valve (40%) and atheromatous plaque (48%) specimens, whereas the quantities of all other tested bacterial species, including several related to periodontitis, were negligible in the cardiovascular samples. These results indicate that S. mutans is a possible causative agent of cardiovascular disease.