The MDCK cell line is made up of populations of cells with diverse resistive and transport properties
The MDCK cell line is made up of populations of cells with diverse resistive and transport properties
复制标题
DOI：
10.1054/tice.2000.0135
复制
发表时间：
2000-10-01
期刊：
影响因子：
2.6
通讯作者：
Arthur, JM
中科院分区：
文献类型：
--
作者：
Arthur, JM
MDCK 细胞系已被用作肾远曲小管的模型。当在渗透性支持物上生长时，细胞形成紧密连接并运输钠和钙。然而，不同的研究人员获得了截然不同的抗性和转运值。由于 MDCK 细胞系具有遗传异质性，观察到差异的一个潜在原因是使用不同的细胞群。已使用稀释技术获得 11 个 MDCK 细胞克隆。克隆表现出一系列抗性（300-4000 Omega cm(2)）、净Ca2+转运（3.2-81 pmol /cm(2)/min）和净Na+转运（0.4-6.0 nmol/cm(2)/min）值。为了确定克隆细胞系的特征是否随着传代次数的增加而变化，在相对早期和晚期的传代次数中测量了抗性。六个细胞系中的两个细胞系中的电阻随着传代次数的增加而增加，而一个细胞系的电阻则降低。这些研究表明，先前研究之间的差异可能是由于细胞群、传代后的时间长度和传代次数的差异造成的。克隆细胞系应该为研究肾远曲小管模型中的离子转运和转运调节机制提供有用的工具，(C) 2000 Harcourt Publishers Ltd.。
The MDCK cell line has been used as a model for the renal distal tubule. When grown on permeable supports, the cells form tight junctions and transport sodium and calcium. Widely different values have, however, been obtained for resistance and transports by different investigators. Since the MDCK cell line is genetically heterogeneous, one potential reason for observed differences is the use of different populations of cells, Dilutional techniques have been used to obtain eleven MDCK cell clones. The clones exhibit a range of values for resistance (300-4000 Omega cm(2)), net Ca2+ transport (3.2-81 pmol /cm(2)/min) and net Na+ transport (0.4-6.0 nmol/cm(2)/min), To determine if the characteristics of clonal cell lines changed with increasing passage number, resistance was measured at relatively early and late passage number. Resistance increased with increasing passage number in two of six lines and decreased in one, These studies demonstrate that differences between previous studies may be due to differences in cell population, length of time since passage, and passage number, Clonal cell lines should provide a useful tool to investigate ion transport and mechanisms of regulation of transport in this model of renal distal tubule, (C) 2000 Harcourt Publishers Ltd.