A DNA-based West Nile virus replicon elicits humoral and cellular immune responses in mice
A DNA-based West Nile virus replicon elicits humoral and cellular immune responses in mice
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基于 DNA 的西尼罗河病毒复制子在小鼠体内引发体液和细胞免疫反应
DOI：
10.1016/j.jviromet.2011.08.018
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发表时间：
2011-12-01
影响因子：
3.1
通讯作者：
Qin, Cheng-Feng
中科院分区：
文献类型：
--
作者：
Cao, Fei;Li, Xiao-Feng;Qin, Cheng-Feng
虽然已经描述了自我复制的非传染性亚基因组黄病毒复制子，但它们大多数是受Sp6或T7启动子控制的RNA转录物。本研究以西尼罗河病毒（WNV）为模型，在微小巨细胞病毒（CMV）即刻早期启动子的控制下构建了一系列基于dna的报告复制子，功能分析表明这些报告复制子在哺乳动物细胞中能够高效复制。用基于dna的西尼罗河病毒复制子免疫小鼠，可诱导ns1特异性IgG抗体和抗西尼罗河病毒中和抗体。此外，用这种基于dna的西尼罗河病毒复制子免疫可诱导淋巴细胞高水平增殖，并增强ifn - γ的分泌。这些结果表明，这种基于dna的复制子可以诱导小鼠的体液和细胞免疫反应，这表明这种基于dna的复制子可以作为疫苗开发和蛋白质表达的有用平台。(C) 2011 Elsevier B.V.版权所有
While self-replicating, non-infectious subgenomic flavivirus replicons have been described, most of them are RNA transcripts under the control of an Sp6 or T7 promoter. In this study, using West Nile virus (WNV) as a model, a series of DNA-based reporter replicons under the control of a minimal cytomegalovirus (CMV) immediate-early promoter were constructed, and functional analysis showed that these reporter replicons replicate efficiently in mammalian cells. When the DNA-based WNV replicon was used to immunize mice, NS1-specific IgG antibodies and anti-WNV neutralizing antibodies were both induced. Additionally, immunization with this DNA-based WNV replicon induced high levels of lymphocyte proliferation and enhanced the secretion of IFN-gamma. These results suggest that this type of DNA-based replicon can induce humoral and cellular immune responses in mice, indicating that this type of DNA-based replicon may serve as a useful platform for vaccine development and protein expression. (C) 2011 Elsevier B.V. All rights reserved.