Er81 is expressed in a subpopulation of layer 5 neurons in rodent and primate neocortices
Er81 is expressed in a subpopulation of layer 5 neurons in rodent and primate neocortices
复制标题
DOI：
10.1016/j.neuroscience.2005.08.075
复制
发表时间：
2006-12
期刊：
影响因子：
3.3
通讯作者：
H. Yoneshima;S. Yamasaki;C. Voelker;Z. Molnár;E. Christophe;E. Audinat;M. Takemoto;M. Nishiwaki;S. Tsuji;I. Fujita;N. Yamamoto
中科院分区：
文献类型：
--
作者：
H. Yoneshima;S. Yamasaki;C. Voelker;Z. Molnár;E. Christophe;E. Audinat;M. Takemoto;M. Nishiwaki;S. Tsuji;I. Fujita;N. Yamamoto
层状结构是哺乳动物中枢神经系统的基本细胞结构，也是新皮层的显著特征。ER 81是一种转录因子，最近已被用作新皮层第5层细胞的标记物。我们进一步追踪ER 81的分布以研究脑中ER 81表达细胞的身份。Er 81转录本在第5层整个宽度上分散的锥体细胞亚群中表达。在大鼠皮层中，Er 81转录本在E15时首先在脑室区检测到，在婴儿和青少年阶段仍然在推定的预期第5层神经元中表达。成人第5层神经元中的ER 81表达亚群不与投射靶点的表型分离。通过逆行标记结合免疫组织化学或逆转录-聚合酶链反应分析，我们发现ER 81表达在几乎所有的第5层神经元投射到脊髓或上级丘，而只有三分之一的第5层神经元投射到对侧皮质。Er 81在P2日本猕猴的第5层神经元中也检测到，但在成年猴皮质中未检测到。这些研究结果表明，由分子标准定义的神经元类别不一定与由解剖学标准定义的神经元类别分离，ER 81参与第5层投射神经元的子集的细胞分化，并且这种机制在啮齿动物和灵长类动物中是保守的。
Laminar organization is a fundamental cytoarchitecture in mammalian CNS and a striking feature of the neocortex. ER81, a transcription factor, has recently been utilized as a marker of cells in the layer 5 of the neocortex. We further pursued the distribution of ER81 to investigate the identity of the ER81-expressing cells in the brain. Er81 transcript was expressed in a subset of pyramidal cells that were scattered throughout the entire width of layer 5. In the rat cortex, Er81 transcripts were first detected in the ventricular zone at E15, remained expressed in putative prospective layer 5 neurons during infant and juvenile stages. The ER81-expressing subpopulation in adult layer 5 neurons did not segregate with the phenotypes of the projection targets. By retrograde labeling combined with immunohistochemistry or reverse transcription–polymerase chain reaction analysis, we found ER81 expression in nearly all of the layer 5 neurons projecting to the spinal cord or to the superior colliculus, while in only one-third of the layer 5 neurons projecting to the contralateral cortex. Er81 was also detected in layer 5 neurons in a P2 Japanese macaque monkey but not in adult monkey cortices. These findings suggest that a neuron class defined by a molecular criterion does not necessarily segregate with that defined by an anatomical criterion, that ER81 is involved in cell differentiation of a subset of layer 5 projection neurons and that this mechanism is conserved among rodents and primates.