Whole-brain functional imaging at cellular resolution using light-sheet microscopy
Whole-brain functional imaging at cellular resolution using light-sheet microscopy
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DOI：
10.1038/nmeth.2434
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发表时间：
2013-05-01
期刊：
影响因子：
48
通讯作者：
Keller, Philipp J.
中科院分区：
文献类型：
--
作者：
Ahrens, Misha B.;Orger, Michael B.;Keller, Philipp J.
大脑功能依赖于跨越多个大脑区域的大量神经元之间的交流，要充分了解这一点，需要了解中枢神经系统中所有神经元的时变活动。在这里，我们使用光片显微镜来记录活动，通过基因编码的钙指示器GCaMP5G报道，从0.8 Hz的斑马鱼幼虫的整个大脑体积中，在单细胞分辨率下捕获80%以上的所有神经元。为了证明这项技术可以用来揭示大脑中功能明确的回路，我们确定了两组具有相关活动模式的神经元。一个回路由后脑神经元与脊髓神经细胞功能耦合组成。另一组由解剖学上对称的后前脑群组成，左右半脑的活动在20秒的时间尺度上以反相位振荡，并与下橄榄脑同样缓慢的振荡相结合。
Brain function relies on communication between large populations of neurons across multiple brain areas, a full understanding of which would require knowledge of the time-varying activity of all neurons in the central nervous system. Here we use light-sheet microscopy to record activity, reported through the genetically encoded calcium indicator GCaMP5G, from the entire volume of the brain of the larval zebrafish in vivo at 0.8 Hz, capturing more than 80% of all neurons at single-cell resolution. Demonstrating how this technique can be used to reveal functionally defined circuits across the brain, we identify two populations of neurons with correlated activity patterns. One circuit consists of hindbrain neurons functionally coupled to spinal cord neuropil. The other consists of an anatomically symmetric population in the anterior hindbrain, with activity in the left and right halves oscillating in antiphase, on a timescale of 20 s, and coupled to equally slow oscillations in the inferior olive.