Circulating human B and plasma cells. Age-associated changes in counts and detailed characterization of circulating normal CD138- and CD138+ plasma cells
Circulating human B and plasma cells. Age-associated changes in counts and detailed characterization of circulating normal CD138- and CD138+ plasma cells
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DOI：
10.3324/haematol.2009.018689
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发表时间：
2010-06-01
期刊：
影响因子：
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通讯作者：
Perez-Andres, Martin
中科院分区：
文献类型：
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作者：
Caraux, Anouk;Klein, Bernard;Perez-Andres, Martin
B细胞和浆细胞的产生涉及几个器官，它们之间有必要的细胞运输。用多参数流式细胞术对106例健康成人外周血中四种B细胞亚群(幼稚B细胞、幼稚B细胞、记忆性B细胞和浆细胞)进行了详细的表型分析。我们发现CD10、CD27和CD38是亚群标记的最小组合，允许明确识别循环CD19(+)细胞中的未成熟(CD10(+)CD27(-)CD38(+)，6+/-6个/亩L)、幼稚(CD10-CD27-CD38-，12590个/亩L)、记忆性B淋巴细胞(CD10-CD27+CD38(+)，58+/-42个/亩L)和浆细胞(CD10(-)CD27(++)CD38(++)，2.1+/-2.1个/亩L)。在这四个亚群中，只有记忆B淋巴细胞和浆细胞随着年龄的增长而减少，无论是相对计数还是绝对计数。循环浆细胞分裂为CD138(-)(57+/-12%)和CD138(+)(43+/-12%)细胞，后者表现出更成熟的表型：表面免疫球蛋白缺失，CD45阳性率较低，胞浆免疫球蛋白CD38和CD27含量较高。与B淋巴细胞不同，这两类浆细胞均为Ki-67(+)，且CXCR4表达较弱。
Generation of B and plasma cells involves several organs with a necessary cell trafficking between them. A detailed phenotypic characterization of four circulating B-cell subsets (immature-, naive-, memory- B-lymphocytes and plasma cells) of 106 healthy adults was realized by multiparametric flow cytometry. We show that CD 10, CD27 and CD38 is the minimal combination of subsetting markers allowing unequivocal identification of immature (CD10(+)CD27(-)CD38(+), 6+/-6 cells/mu L), naive (CD10-CD27-CD38-, 125 90 cells/mu L), memory B lymphocytes (CD10-CD27+CD38(+), 58+/-42 cells/mu L), and plasma cells (CD10(-)CD27(++)CD38(++), 2.1+/-2.1 cells/mu L) within circulating CD19(+) cells. From these four subsets, only memory B lymphocytes and plasma cells decreased with age, both in relative and absolute counts. Circulating plasma cells split into CD138(-) (57+/-12%) and CD138(+) (43+/-12%) cells, the latter displaying a more mature phenotypic profile: absence of surface immunoglobulin, lower CD45 positivity and higher amounts of cytoplasmic immunoglobulin, CD38 and CD27. Unlike B lymphocytes, both populations of plasma cells are KI-67(+) and show weak CXCR4 expression.