PALMITOYL-ACYL CARRIER PROTEIN (ACP) THIOESTERASE AND THE EVOLUTIONARY ORIGIN OF PLANT ACYL-ACP THIOESTERASES
PALMITOYL-ACYL CARRIER PROTEIN (ACP) THIOESTERASE AND THE EVOLUTIONARY ORIGIN OF PLANT ACYL-ACP THIOESTERASES
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DOI：
10.1105/tpc.7.3.359
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发表时间：
1995-03-01
期刊：
影响因子：
11.6
通讯作者：
VOELKER, TA
中科院分区：
文献类型：
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作者：
JONES, A;DAVIES, HM;VOELKER, TA
酰基-酰基载体蛋白（ACP）硫酯酶在植物脂肪酸从头合成过程中的链终止和两种脂质生物合成途径之间的碳通量通道中起着至关重要的作用。我们发现在高等植物中存在两类不同但相关的硫酯酶基因，称为FatA和Fate，它们的进化分歧似乎是古老的。FatA编码已经描述的18：1-ACP硫酯酶。相反，Fate代表编码硫酯酶，其偏好具有饱和酰基的酰基-ACP。我们意外地从细萼距花种子中获得了16：0-ACP硫酯酶cDNA，其主要积累8：0和10：0。在非种子组织中发现了16：0硫酯酶转录物，并且在转基因甘蓝型油菜中的表达导致产生富含16：0的油。我们提出的证据表明，这种类型的脂肪基因是古老的，普遍存在于植物中，专门的植物中链硫酯酶已经独立于这样的酶在被子植物进化过程中的几次，此外，无处不在的18：1-ACP硫酯酶似乎是一个衍生物的16：0硫酯酶。
Acyl-acyl carrier protein (ACP) thioesterases play an essential role in chain termination during de novo fatty acid synthesis and in the channeling of carbon flux between the two lipid biosynthesis pathways in plants. We have discovered that there are two distinct but related thioesterase gene classes in higher plants, termed FatA and Fate, whose evolutionary divergence appears to be ancient. FatA encodes the already described 18:1-ACP thioesterase. In contrast, Fate representatives encode thioesterases preferring acyl-ACPs having saturated acyl groups. We unexpectedly obtained a 16:0-ACP thioesterase cDNA from Cuphea hookeriana seed, which accumulate predominantly 8:0 and 10:0. The 16:0 thioesterase transcripts were found in non-seed tissues, and expression in transgenic Brassica napus led to the production of a 16:0- rich oil. We present evidence that this type of Fats gene is ancient and ubiquitous in plants and that specialized plant medium-chain thioesterases have evolved independently from such enzymes several times during angiosperm evolution, Also, the ubiquitous 18:1-ACP thioesterase appears to be a derivative of a 16:0 thioesterase.