High diagnostic performance of ELISA detection of antibodies to citrullinated antigens in rheumatoid arthritis
High diagnostic performance of ELISA detection of antibodies to citrullinated antigens in rheumatoid arthritis
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DOI：
10.1080/03009740310003677
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发表时间：
2003-08-01
影响因子：
2.1
通讯作者：
Yamamoto, K
中科院分区：
文献类型：
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作者：
Suzuki, K;Sawada, T;Yamamoto, K
目的：探讨抗环瓜氨酸肽抗体（anti-CCP）和抗聚丝蛋白抗体（AFA）对类风湿关节炎（RA）的诊断价值，并与RF和基质金属蛋白酶-3 （MMP-3）进行比较。方法：采用第二代酶联免疫吸附试验（ELISA）试剂盒检测抗ccp。我们构建了重组人聚丝蛋白，用人多肽精氨酸脱亚胺酶体外瓜氨酸化，并将其作为AFA-ELISA包被抗原。共有549名RA患者和208名其他风湿病患者被纳入研究。结果：抗ccp（88.9%）和AFA（94.7%）特异性优于RF（81.7%）和MMP-3（49.5%）。抗ccp的敏感性（87.6%）优于其他方法。然而，AFA的敏感性（68.7%）低于RF（69.8%）和MMP-3（75.7%）。anti-CCP和AFA的受试者工作特征曲线比RF和MMP-3更靠近左上角，且AFA和anti-CCP的曲线下面积（AUC）明显更大。此外，anti-CCP的AUC显著大于AFA。结论：ELISA检测瓜氨酸化抗原抗体，特别是第二代抗ccp抗体，比RF等其他检测方法具有更高的鉴别能力，有助于临床诊断RA。
Objective: We investigated the rheumatoid arthritis (RA) diagnostic performances of anti-cyclic citrullinated peptide antibody (anti-CCP) and antifilaggrin antibody (AFA) in comparison with RF and matrix metalloproteinase-3 (MMP-3).Methods: We used a second generation enzyme-linked immunosorbent assay (ELISA) kit for the detection of anti-CCP. We constructed recombinant human filaggrin, which was citrullinated in vitro by human peptidylarginine deiminase, and subsequently used it as the coating antigen for AFA-ELISA. A total of 549 RA patients and 208 other rheumatic disease patients were included in the study.Results: The specificities of anti-CCP (88.9%) and AFA (94.7%) were superior to those of RF (81.7%) and MMP-3 (49.5%). The sensitivity of anti-CCP (87.6%) was superior to all others. However, the sensitivity of AFA (68.7%) was inferior to those of RF (69.8%) and MMP-3 (75.7%). Furthermore, receiver operating characteristic curves of anti-CCP and AFA passed closer to the upper left corner than those of RF and MMP-3, and the areas under the curves (AUC) of AFA and anti-CCP were significantly larger. In addition, the AUC of anti-CCP was significantly larger than that of AFA.Conclusion: ELISA detection of antibodies to citrullinated antigens, especially a second generation anti-CCP, showed higher discriminative ability than other assays, including RF, and would be useful to aid the diagnosis of RA in clinical practice.