Expression from DIF1-motif promoters of hetR and patS is dependent on HetZ and modulated by PatU3 during heterocyst differentiation
Expression from DIF1-motif promoters of hetR and patS is dependent on HetZ and modulated by PatU3 during heterocyst differentiation
复制标题
hetR 和 patS 的 DIF1 基序启动子的表达依赖于 HetZ，并在异形细胞分化过程中受 PatU3 调节
DOI：
10.1371/journal.pone.0232383
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发表时间：
2020-07-23
期刊：
影响因子：
3.7
通讯作者：
Xu, Xudong
中科院分区：
文献类型：
--
作者：
Du, Yaru;Zhang, He;Xu, Xudong
HetR和PatS/ patx衍生肽是蓝藻异囊形成细胞分化和模式的激活剂和扩散抑制剂。HetR通过HetR识别位点调控靶基因。然而，在异囊分化早期上调的一些基因（如patS/patX）具有DIF1（或DIF+）基序（TCCGGA）启动子，而不是hetr识别位点；hetR同时具有两个预测的调控元件。HetR如何控制DIF1基序启动子的异囊特异性表达仍有待回答。本研究证明，hetR、patS和patX的DIF1基序启动子的表达更直接依赖于hetZ， hetR通过hetR识别位点调控hetZ基因。hetR上游的hetR结合位点不需要hetR的自动调节。与HetZ相互作用的PatU3 （PatU的3′部分）可以调节hetR、HetZ和patS的表达。这些发现有助于理解hetR， hetZ-patU和patS/patX在一大群多细胞蓝藻中的相互调节。
HetR and PatS/PatX-derived peptides are the activator and diffusible inhibitor for cell differentiation and patterning in heterocyst-forming cyanobacteria. HetR regulates target genes via HetR-recognition sites. However, some genes (such as patS/patX) upregulated at the early stage of heterocyst differentiation possess DIF1 (or DIF+) motif (TCCGGA) promoters rather than HetR-recognition sites; hetR possesses both predicted regulatory elements. How HetR controls heterocyst-specific expression from DIF1 motif promoters remains to be answered. This study presents evidence that the expression from DIF1 motif promoters of hetR, patS and patX is more directly dependent on hetZ, a gene regulated by HetR via a HetR-recognition site. The HetR-binding site upstream of hetR is not required for the autoregulation of hetR. PatU3 (3' portion of PatU) that interacts with HetZ may modulate the expression of hetR, hetZ and patS. These findings contribute to understanding of the mutual regulation of hetR, hetZ-patU and patS/patX in a large group of multicellular cyanobacteria.