Expression and regulation of histidine decarboxylase mRNA expression in the uterus during pregnancy in the mouse.
Expression and regulation of histidine decarboxylase mRNA expression in the uterus during pregnancy in the mouse.
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小鼠妊娠期间子宫内组氨酸脱羧酶 mRNA 表达及调控。
DOI：
10.1006/cyto.2000.0667
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Dileepan,KN
中科院分区：
文献类型：
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作者：
Wood,GW;Hausmann,EH;Choudhuri,R;Dileepan,KN
据推测，荷尔蒙调节的组胺产生在子宫植入准备过程中起着重要作用。组氨酸脱羧酶(HDC)是组胺产生的限速酶。本研究旨在确定妊娠期间HDC基因在小鼠子宫内的表达。小鼠着床前子宫中HDC mRNA表达水平较高，峰值出现在第4天，植入后子宫中也有较高水平的HDC mRNA表达。为了确定HDC基因是否受促炎症细胞因子的调节，比较了HDC基因在围着床期子宫内的IL-1α(IL-1α)、IL-1β、巨噬细胞趋化蛋白-1(MCP-1)和RANTES(受激活调节，T细胞正常表达和分泌)的表达。IL-1β、单核细胞趋化蛋白-1和RANTESα在1~2天和4~5天在子宫中表达升高，IL-1 mRNA在1~2天和5~7天表达增加。孕酮刺激子宫内HDC基因的表达。子宫内细胞因子/趋化因子mRNA也受激素调节。这一数据使得这些促炎细胞因子中的一个或多个可能参与调节子宫内HDC mRNA的产生。在小鼠假孕模型中，重组IL-1α、IL-1β、单核细胞趋化蛋白-1和RANTES均不能诱导植入前子宫HDC mRNA的表达。同时，IL-1β可诱导上述四种细胞因子/趋化因子的表达。尽管这些细胞因子在怀孕期间也在子宫中产生，并受到激素的调节，但这些细胞因子都没有诱导子宫内HDC mRNA的表达。结果提示，孕酮参与了着床前子宫HDC基因表达的调节，而IL-1α/β、单核细胞趋化蛋白-1和RANTES在炎症过程中调节组胺合成的作用不明显。
It has been hypothesized that hormonally regulated histamine production plays a role in preparation of the uterus for implantation. Histidine decarboxylase (HDC) is the rate-limiting enzyme for histamine production. The current study was designed to determine intrauterine expression of HDC mRNA expression during pregnancy in the mouse. High levels of HDC mRNA expression were observed in the preimplantation mouse uterus with peak expression occurring on day 4. High levels of HDC mRNA expression were also detected in the post-implantation uterus. In an effort to determine whether HDC mRNA is regulated by pro-inflammatory cytokines, the HDC mRNA pattern was compared to intrauterine expression of mRNA's for interleukin-1α (IL-1α), IL-1β, macrophage chemotactic protein-1 (MCP-1) and RANTES (regulated on activation, normal T expressed and secreted) during the peri-implantation period. IL-1β, MCP-1 and RANTES mRNA levels were increased in the uterus on days 1–2 and on days 4–5. Increased expression of IL-1α mRNA was observed on days 1–2 and days 5–7. There was no clear relationship between HDC mRNA expression and cytokine/chemokine mRNA expression. Progesterone-stimulated intrauterine expression of HDC mRNA. Intrauterine cytokine/chemokine mRNA was also hormonally regulated. This data allowed the possibility that one or more of these pro-inflammatory cytokines could be involved in regulating intrauterine HDC mRNA production. Recombinant IL-1α, IL-1β, MCP-1 and RANTES all failed to induce HDC mRNA expression in the preimplantation uterus in a mouse pseudopregnancy model. At the same time, IL-1β induced the expression of mRNA for each of the four cytokines/chemokines. Despite the fact that these were also produced in the uterus during pregnancy and were hormonally regulated, none of these cytokines induced intrauterine HDC mRNA expression. The data suggest that progesterone is involved in the regulation of HDC mRNA expression in the preimplantation uterus, but IL-1α/β, MCP-1 and RANTES, which have been reported to regulate histamine synthesis during inflammatory processes, do not appear to play a role.