Genetic heterogeneity in human T-cell leukemia/lymphoma virus type II.
Genetic heterogeneity in human T-cell leukemia/lymphoma virus type II.
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人类 T 细胞白血病/淋巴瘤病毒 II 型的遗传异质性。
DOI：
10.1128/jvi.67.3.1175-1184.1993
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发表时间：
1993
影响因子：
5.4
通讯作者：
Glaser,JB
中科院分区：
文献类型：
--
作者：
Dube,DK;Sherman,MP;Saksena,NK;Bryz-Gornia,V;Mendelson,J;Love,J;Arnold,CB;Spicer,T;Dube,S;Glaser,JB
用引物对SK 110/SK 111，通过聚合酶链反应（PCR）成功地扩增了来自17个不同的感染人类T细胞淋巴瘤/白血病病毒II型（HTLV-II）的个体的外周血单个核细胞的DNA。该引物对在所有灵长类T细胞淋巴瘤病毒（PTLV）的pol基因中是保守的，并且侧翼是140 bp的DNA片段，当用于比较分析时，其反映了PTLV基因组之间的相对多样性程度。这些扩增的140 bp聚合酶片段的克隆，测序和系统发育比较表明，至少有两个不同的HTLV-II在西半球的遗传亚株。通过使用对PTLV基因组中不同区域具有特异性的10个额外引物对-探针组进行PCR、克隆和测序，确认了选定分离株的这些数据。从塞米诺尔、瓜伊米和托巴斯印第安人分离的HTLV-II属于HTLV-II的新亚株，而原型MoT分离物定义了原始亚株。HTLV-II新世界毒株之间的多样性大于HTLV-I新世界毒株之间的多样性。事实上，来自西半球的HTLV-II毒株之间的异质性与来自世界各地（包括日本、非洲和巴布亚新几内亚）的HTLV-I和猴T细胞淋巴瘤/白血病病毒I型分离株中观察到的异质性相似。鉴于这些地理和人类学的考虑，并假设类似的突变率和选择力之间的PTLV，这些数据表明，HTLV-II已经存在了很长一段时间，在土著美洲印第安人人口或HTLV-II隔离引入新大陆比HTLV-I株引入新大陆的异质性。
DNA from the peripheral blood mononuclear cells of 17 different individuals infected with human T-cell lymphoma/leukemia virus type II (HTLV-II) was successfully amplified by the polymerase chain reaction (PCR) with the primer pair SK110/SK111. This primer pair is conserved among the pol genes of all primate T-cell lymphoma viruses (PTLV) and flanks a 140-bp fragment of DNA which, when used in comparative analyses, reflects the relative degree of diversity among PTLV genomes. Cloning, sequencing, and phylogenetic comparisons of these amplified 140-bp pol fragments indicated that there are at least two distinct genetic substrains of HTLV-II in the Western Hemisphere. These data were confirmed for selected isolates by performing PCR, cloning, and sequencing with to 10 additional primer pair-probe sets specific for different regions throughout the PTLV genome. HTLV-II isolates from Seminole, Guaymi, and Tobas Indians belong in the new substrain of HTLV-II, while the prototype MoT isolate defines the original substrain. There was greater diversity among HTLV-II New World strains than among HTLV-I New World strains. In fact, the heterogeneity among HTLV-II strains from the Western Hemisphere was similar to that observed in HTLV-I and simian T-cell lymphoma/leukemia virus type I isolates from around the world, including Japan, Africa, and Papua New Guinea. Given these geographic and anthropological considerations and assuming similar mutation rates and selective forces among the PTLV, these data suggest either that HTLV-II has existed for a long time in the indigenous Amerindian population or that HTLV-II isolates introduced into the New World were more heterogeneous than the HTLV-I strains introduced into the New World.