A novel NAC transcription factor from Suaeda liaotungensis K. enhanced transgenic Arabidopsis drought, salt, and cold stress tolerance
A novel NAC transcription factor from Suaeda liaotungensis K. enhanced transgenic Arabidopsis drought, salt, and cold stress tolerance
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辽东碱蓬新型 NAC 转录因子增强转基因拟南芥的干旱、盐和冷胁迫耐受性
DOI：
10.1007/s00299-014-1602-y
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发表时间：
2014-05-01
影响因子：
6.2
通讯作者：
Li, Qiu-li
中科院分区：
文献类型：
--
作者：
Li, Xiao-lan;Yang, Xing;Li, Qiu-li
SL NAC1作为一种胁迫响应NAC蛋白参与脱落酸依赖的信号转导途径，增强转基因拟南芥对干旱、盐和低温胁迫的耐受性。NAC(NAM，ATAF1，2，CUC2)转录因子是植物特有的转录因子家族中最大的一类，参与植物的各种生长发育过程和对环境胁迫的响应调控。本研究通过聚合酶链式反应从辽东碱蓬中克隆了一个全长的NAC基因，命名为SlNAC1，并通过在转基因拟南芥中的过表达来研究其功能。SlNAC1含有一个NAC保守结构域。干旱、高盐、冷(4℃)胁迫和脱落酸诱导了其在辽东沙棘中的表达。在洋葱表皮细胞的亚细胞定位实验表明，SlNAC1定位于细胞核。酵母单杂交实验表明，SlNAC1具有转录激活功能。与野生型拟南芥相比，转slNAC1基因拟南芥在干旱胁迫下表现出较高的存活率和较低的失水率；在盐胁迫下表现出较高的发芽率、较高的存活率和较低的根抑制率；在冷胁迫下表现出较高的存活率；在脱落酸处理下表现出较低的发芽率和生根抑制率。这些结果表明，SlNAC1作为一种胁迫响应NAC蛋白参与了脱落酸依赖的信号转导途径，可能在转基因育种中提高作物的非生物抗逆性方面具有潜在的应用价值。
Sl NAC1 functions as a stress-responsive NAC protein involved in the abscisic acid-dependent signaling pathway and enhances transgenic Arabidopsis drought, salt, and cold stress tolerance. NAC (NAM, ATAF1, 2, CUC2) transcription factors constitute the largest families of plant-specific transcription factors, known to be involved in various growth or developmental processes and in regulation of response to environmental stresses. However, only little information regarding stress-related NAC genes is available in Suaeda liaotungensis K. In this study, we cloned a full-length NAC gene (1,011 bp) named SlNAC1 using polymerase chain reaction from Suaeda liaotungensis K. and investigated its function by overexpression in transgenic Arabidopsis. SlNAC1 contains an NAC-conserved domain. Its expression in S. liaotungensis was induced by drought, high-salt, and cold (4 °C) stresses and by abscisic acid. Subcellular localization experiments in onion epidermal cells indicated that SlNAC1 is localized in the nucleus. Yeast one-hybrid assays showed that SlNAC1 functions as a transcriptional activator. SlNAC1 transgenic Arabidopsis displayed a higher survival ratio and lower rate of water loss under drought stress; a higher germination ratio, higher survival ratio, and lower root inhibition rate under salt stress; a higher survival ratio under cold stress; and a lower germination ratio and root inhibition rate under abscisic acid treatment, compared with wild-type Arabidopsis. These results suggested that SlNAC1 functions as a stress-responsive NAC protein involved in the abscisic acid-dependent signaling pathway and may have potential applications in transgenic breeding to enhance crops' abiotic stress tolerances.