ELECTRO-PHYSIOLOGICAL CORRELATES OF PULSATILE GONADOTROPIN-RELEASE IN RATS
ELECTRO-PHYSIOLOGICAL CORRELATES OF PULSATILE GONADOTROPIN-RELEASE IN RATS
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DOI：
10.1159/000123356
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发表时间：
1982-01-01
影响因子：
4.1
通讯作者：
HAYASHI, R
中科院分区：
文献类型：
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作者：
KAWAKAMI, M;UEMURA, T;HAYASHI, R
为了揭示去卵巢大鼠脉冲性LH [黄体生成素]释放的电生理相关性，记录了轻度麻醉雌性大鼠弓状核（ARC）、内侧视前区、腹内侧核、下丘脑前区和其他脑区的多单位活动（MUA）的变化。在实验前至少7周切除大鼠卵巢。在记录过程中，每隔10分钟从心导管中抽取血样[0.1 ml]，通过放射免疫测定法测定血浆LH浓度的变化。持续静脉输注硫喷妥钠时LH浓度呈搏动性变化，而皮层脑电图在整个实验过程中保持稳定。ARC中部的放电率（8次记录）与血浆LH浓度的增加平行变化，并在LH分泌增加之前突然升高。每分钟的MUA峰放电数增加到基础水平的1.5-5倍。MUA在LH升高前2-9 min开始升高，并持续1-5 min。ARC区（14个记录）和其它区（54个记录）的放电频率与LH升高不同步。由于MUA的这些变化局限于ARC中，并且未反映在皮层EEG中，因此它们可能是与涉及整个大脑的全身效应不同的特定局部变化。MUA的这些极端增加似乎是由于分泌LHRH或控制LHRH分泌的神经元的高活性。
To reveal the electrophysiological correlates of pulsatile LH [luteinizing hormone] release in ovariectomized rats, changes in multiple unit activity (MUA) in the arcuate nucleus (ARC), the medial preoptic area, the ventromedial nulceus, the anterior hypothalamic area and other areas of the brain were recorded in lightly anesthetized female rats. Rats were ovariectomized at least 7 wk prior to the experiment. Blood samples [0.1-ml] were withdrawn from a cardiac catheter at 10-min intervals during the recording to measure the changes in the plasma LH concentration by radioimmunoassay. The LH concentration showed pulsatile changes under continuous i.v. infusion of thiopental sodium while cortical EEG was stable throughout the experiment. The firing rate in the middle part of the ARC (8 recordings) changed in parallel with the increase in the plasma LH concentration and was abruptly raised just prior to an increase in LH secretion. The number of MUA spike discharges per minute increased to 1.5-5 times that of the basal level. MUA started to increase 2-9 min prior to the LH rise and the high level of MUA lasted for 1-5 min. The firing rate in the ARC (14 recordings) and in other areas (54 recordings) recorded did not change periodically in parallel with the increase in LH. As these changes in MUA were localized in the ARC and were not reflected in cortical EEG, they might be specific local changes distinguished from generated systemic effects involving the whole brain. These extreme increases in MUA seem to be due to the high activity either of neurons that secrete LHRH or those that control the secretion of LHRH.