Antigenic variability of Aspergillus fumigatus strains.
Antigenic variability of Aspergillus fumigatus strains.
复制标题
烟曲霉菌株的抗原变异性。
DOI：
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发表时间：
1977
期刊：
影响因子：
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通讯作者：
Falk Mj
中科院分区：
文献类型：
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作者：
V. Kurup;Jordan N. Fink;G. Scribner;Falk Mj
评估了培养基、培养温度和培养物的连续振荡对烟曲霉抗原的反应性和产量的影响。结果发现，AOAC 培养基优于 Czapek 培养基，并且摇动培养比静止培养产生更好的结果。然而，来自于 AOAC 培养基中在 30 摄氏度下孵育 3 周的固定培养物的抗原与从 2 周大的摇动培养物中获得的抗原同样好。采用琼脂凝胶双扩散法，选取 11 株烟曲霉抗原，对 33 份不同类型曲霉病患者血清和 35 份正常对照血清中的抗体活性进行检测。结果显示，各个抗原的反应性从 42% 到 87% 不等，表明可以使用来自不止一种烟曲霉菌株的抗原。通过二维免疫电泳研究菌株之间的交叉反应性。讨论了聚丙烯酰胺凝胶电泳和交叉免疫电泳在曲霉菌抗原质量保证中的应用。
The effect of culture media, temperature of incubation, and continuous shaking of cultures, on the reactivity and yield of antigens of Aspergillus fumigatus were evaluated. It was found that AOAC medium was superior to Czapek medium and shake cultures yielded better results than stationary cultures. However, antigens from stationary cultures in AOAC medium incubated at 30 degrees C for 3 weeks were equally as good as antigens obtained from 2-week-old shake cultures. Antigens from 11 selected strains of Aspergillus fumigatus were used to test antibody activity in 33 sera from patients with various forms of aspergillosis and 35 normal controls by the agar gel double-diffusion method. The results showed that the reactivity of individual antigens varied from 42 to 87%, indicating that antigens from more than one strain of Aspergillus fumigatus may be used. The cross-reactivity between strains were studied by two-dimensional immunoelectrophoresis. The use of polyacrylamide gel electrophoresis and crossed immunoelectrophoresis in the quality assurance of Aspergillus antigens is discussed.