A proteomic analysis of Helicoverpa armigera adults after exposure to UV light irradiation
A proteomic analysis of Helicoverpa armigera adults after exposure to UV light irradiation
复制标题
紫外光照射后棉铃虫成虫的蛋白质组学分析
DOI：
10.1016/j.jinsphys.2009.11.015
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发表时间：
2010-04-01
影响因子：
2.2
通讯作者：
Lei, Chao-Liang
中科院分区：
文献类型：
--
作者：
Meng, Jian-Yu;Zhang, Chang-Yu;Lei, Chao-Liang
紫外线（UV）灯（黑光），其发射的紫外线在320-400纳米范围内，已在世界范围内用于捕虫。为了更好地了解棉铃虫成虫对紫外线照射的反应，我们进行了比较蛋白质组学分析。3日龄成虫暴露于紫外光下1小时，提取总蛋白并进行双向凝胶电泳分离。可重复检测到1200多个蛋白点，其中12个较丰富，21个较不丰富。质谱分析和数据库检索帮助我们鉴定了29种差异丰富的蛋白质。鉴定出的蛋白质可分为信号转导、RNA加工、蛋白质加工、应激反应、代谢和细胞骨架结构等功能群。本研究首次分析了趋光性昆虫在紫外光照射条件下的差异表达蛋白，为了解昆虫对紫外光照射胁迫的适应机制提供了新的思路。2009爱思唯尔有限公司版权所有。
Ultraviolet (UV) light (blacklight), which emits UV in the range of 320-400 nm, has been used worldwide in light trapping of insect pests. To gain a better understanding of the response of Helicoverpa armigera adults to UV light irradiation, we carried out a comparative proteomic analysis. Three-day-old adults were exposed to UV light for 1 h. Total proteins were extracted and separated by two-dimensional gel electrophoresis. More than 1200 protein spots were reproducibly detected, including 12 that were more abundant and 21 less abundant. Mass spectrometry analysis and database searching helped us to identify 29 differentially abundant proteins. The identified proteins were categorized into several functional groups including signal transduction, RNA processing, protein processing, stress response, metabolisms, and cytoskeleton structure, etc. This study is the first analysis of differentially expressed proteins in phototactic insects under UV light irradiation conditions and gives new insights into the adaptation mechanisms responsive to UV light irradiation stress. (C) 2009 Elsevier Ltd. All rights reserved.