DIAZEPAM-BINDING INHIBITOR - A NEUROPEPTIDE LOCATED IN SELECTED NEURONAL POPULATIONS OF RAT-BRAIN
DIAZEPAM-BINDING INHIBITOR - A NEUROPEPTIDE LOCATED IN SELECTED NEURONAL POPULATIONS OF RAT-BRAIN
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DOI：
10.1126/science.3892688
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发表时间：
1985-01-01
期刊：
影响因子：
56.9
通讯作者：
GUIDOTTI, A
中科院分区：
文献类型：
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作者：
ALHO, H;COSTA, E;GUIDOTTI, A
鉴定了大鼠脑的内源性多肽，其能够置换1，4-苯并二氮杂卓和β-苯并二氮杂卓的3-羧酸衍生物的酯。从它们的特异性突触结合位点释放咔啉。这种多肽被称为地西泮结合抑制剂（DBI）。DBI脑室注射在啮齿类动物eleventually proconflict反应和拮抗苯二氮卓类药物的抗冲突行动。针对该肽的抗血清针对其氨基末端附近的免疫决定簇，使得有可能检测、测量和研究该肽在大鼠脑中的神经元定位。在大鼠大脑皮层中，DBI免疫反应性位于非GABA能神经元中;然而，在小脑和海马中，它也可能存在于GABA能神经元中。
An endogenous polypeptide of rat brain was identified that is capable of displacing 1,4-benzodiazepines and the esters of the 3-carboxylic acid derivatives of .beta.-carbolines from their specific synaptic binding sites. This polypeptide was termed diazepam-binding inhibitor (DBI). DBI injected intraventricularly in rodents elicits proconflict responses and antagonizes the anticonflict action of benzodiazepines. An antiserum to this peptide, directed toward an immunodeterminant near its amino terminus, makes it possible to detect, measure and study the neuronal location of this peptide in rat brain. In the rat cerebral cortex, DBI immunoreactivity is located in neurons that are not GABAergic; in the cerebellum and hippocampus, however, it might be present also in GABAergic neurons.