Flavonoid treatment of murine macrophages does not alter ingestion or intracellular survival of Listeria monocytogenes.
Flavonoid treatment of murine macrophages does not alter ingestion or intracellular survival of Listeria monocytogenes.
复制标题
类黄酮治疗小鼠巨噬细胞不会改变单核细胞增生李斯特氏菌的摄入或细胞内存活。
DOI：
10.1080/08923970801946873
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发表时间：
2008
影响因子：
3.3
通讯作者：
Czuprynski,CharlesJ
中科院分区：
文献类型：
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作者：
Berryman,Lyndsey;Czuprynski,CharlesJ
最近有报道称芳烃受体（AhR）在小鼠单核细胞增生李斯特菌感染的先天免疫中起作用。在本研究中，作者分析了小鼠巨噬细胞与各种对AhR具有激动剂或拮抗剂活性的黄酮（β-萘黄酮、α-萘黄酮、槲皮素、木犀草素）孵育是否影响l的摄取和存活。monocytogenes。使用RAW264.7和J774小鼠巨噬细胞系和骨髓源性巨噬细胞，作者发现黄酮类化合物处理的巨噬细胞和对照巨噬细胞之间没有显著差异。他们还发现，单独用黄酮类化合物培养的巨噬细胞并没有表现出肿瘤坏死因子(TNF)-α的释放显著增加，TNF -α是抗李斯特菌耐药性的关键细胞因子。
It was recently reported that the aryl hydrocarbon receptor (AhR) plays a role in innate immunity toListeria monocytogenesinfection in mice. In this study the authors analyzed whether incubation of murine macrophages with various flavonoids that have agonist or antagonist activity for the AhR (β-naphthoflavone, α-naphthoflavone, quercetin, luteolin) affect the uptake and survival ofL. monocytogenes. Using the RAW264.7 and J774 murine macrophage cell lines and bone marrow–derived macrophages, the authors found no significant difference between flavonoid-treated and control macrophages. They also found that macrophages incubated with flavonoids alone did not exhibit a significant increase in release of tumor necrosis factor (TNF)-α, a crucial cytokine in anti-Listeriaresistance.