Ex vitro composite plants:: an inexpensive, rapid method for root biology
Ex vitro composite plants:: an inexpensive, rapid method for root biology
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DOI：
10.1111/j.1365-313x.2005.02454.x
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发表时间：
2005-08-01
期刊：
影响因子：
7.2
通讯作者：
Taylor, CG
中科院分区：
文献类型：
--
作者：
Collier, R;Fuchs, B;Taylor, CG
在非模式植物的基因功能分析中，植物转化技术往往是限速步骤。根源农杆菌衍生的复合植物是根生物学家的一个重要工具，它使得对各种转化抗性双子叶植物进行遗传分析成为可能。本报告中描述的新型、快速和廉价的体外培养复合植物的方法代表了现有的复合植物诱导方案的重大进步，这些方案依赖于昂贵和耗时的体外条件。新系统的实用性已通过转基因复合植物根中GFP的表达和RNAi沉默得到验证，并通过RNAi沉默进一步验证了转基因番茄根中植物对植物寄生线虫的内源抗性。汽车复合工厂。本文对该方法的关键参数进行了描述和讨论。
Plant transformation technology is frequently the rate-limiting step in gene function analysis in non-model plants. An important tool for root biologists is the Agrobacterium rhizogenes-derived composite plant, which has made possible genetic analyses in a wide variety of transformation recalcitrant dicotyledonous plants. The novel, rapid and inexpensive ex vitro method for producing composite plants described in this report represents a significant advance over existing composite plant induction protocols, which rely on expensive and time-consuming in vitro conditions. The utility of the new system is validated by expression and RNAi silencing of GFP in transgenic roots of composite plants, and is bolstered further by experimental disruption, via RNAi silencing, of endogenous plant resistance to the plant parasitic nematode Meloidogyne incognita in transgenic roots of Lycopersicon esculentum cv. Motelle composite plants. Critical parameters of the method are described and discussed herein.