Quantitative expression study of four cytokeratins and p63 in squamous cell carcinoma of the tongue: suitability for sentinel node navigation surgery using one-step nucleic acid amplification
Quantitative expression study of four cytokeratins and p63 in squamous cell carcinoma of the tongue: suitability for sentinel node navigation surgery using one-step nucleic acid amplification
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DOI：
10.1136/jclinpath-2011-200085
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发表时间：
2011-10-01
影响因子：
3.4
通讯作者：
Kohno, Naoyuki
中科院分区：
文献类型：
--
作者：
Yamauchi, Kohichi;Fujioka, Yasunori;Kohno, Naoyuki
目标 前哨淋巴结导航手术（SNNS）目前被认为可以提供更好的区域转移分期。为了快速、准确地进行前哨淋巴结分析，使用细胞角蛋白 19 (CK19) 的一步核酸扩增已得到应用，特别是在乳腺癌中。另一方面，最近在头颈癌中报道了含有细胞角蛋白的其他定量逆转录 PCR 靶标。在本报告中，对原发性肿瘤中的 CK19 和 p63 进行了免疫组织化学检查，用作分子标记，并与用于头颈鳞状细胞癌诊断免疫组织化学的细胞角蛋白 903 (CK903)、细胞角蛋白 8/18 (CK8/18) 和细胞角蛋白 (AE1/AE3) 进行比较。 方法 该研究回顾了 17 例 T1/T2、N0 患者选取2002年至2009年间在杏林大学医院接受手术治疗的口腔鳞状细胞癌（UICC），评估肿瘤细胞CK19、CK903、p63、CK8/18和AE1/AE3染色的强度和比例。结果癌细胞胞质中CK19和CK8/18染色呈斑片状，表达较弱。 p63、CK903 和 AE1/AE3 的染色比例大于 CK19 和 CK8/18，但 CK903 的染色强度弱于 p63 和 AE1/AE3。 CK19、CK8/18染色与p63、CK903、AE1/AE3染色总分差异有统计学意义（p
Aims Sentinel node navigation surgery (SNNS) is currently considered to provide better staging of regional metastasis. For rapid and accurate sentinel lymph node analysis, one-step nucleic acid amplification using cytokeratin 19 (CK19) has been applied, particularly in breast cancer. On the other hand, additional quantitative reverse transcription PCR targets containing cytokeratins have been reported recently in head and neck cancer. In this report, CK19 and p63 were immunohistochemically examined in primary tumours for use as molecular markers and were compared with cytokeratin 903 (CK903), cytokeratin 8/18 (CK8/18) and cytokeratin (AE1/AE3), which are used in diagnostic immunohistochemistry for head and neck squamous cell carcinoma.Methods The study reviewed 17 patients with T1/T2, N0 (UICC) oral squamous cell carcinoma of the tongue who were treated surgically at Kyorin University Hospital between 2002 and 2009. The intensity and proportion of tumour cells stained for CK19, CK903, p63, CK8/18 and AE1/AE3 were evaluated.Results CK19 and CK8/18 staining in cytoplasm was patchy among carcinoma cells, indicating weak expression. Staining proportion for p63, CK903 and AE1/AE3 was greater than for CK19 and CK8/18, although staining intensity for CK903 was weaker than for p63 and AE1/AE3. The difference in total score between CK19 and CK8/18 staining and p63, CK903 and AE1/AE3 staining was statistically significant (p