A Species-Wide Inventory of NLR Genes and Alleles in Arabidopsis thaliana
A Species-Wide Inventory of NLR Genes and Alleles in Arabidopsis thaliana
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DOI：
10.1016/j.cell.2019.07.038
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发表时间：
2019-08-22
期刊：
影响因子：
64.5
通讯作者：
Bemm, Felix
中科院分区：
文献类型：
--
作者：
Van de Weyer, Anna-Lena;Monteiro, Freddy;Bemm, Felix
传染病既是自然选择的主要力量，也是农业产量损失的主要原因。在植物中，抗病性通常由核苷酸结合的富含亮氨酸重复序列（NLR）蛋白赋予，NLR蛋白是识别病原体蛋白及其对宿主的影响的细胞内免疫受体。与广泛的平衡和正选择相一致，NLR由植物中最可变的基因家族之一编码，但种内NLR多样性的真实程度尚不清楚。在这里，我们定义了一个几乎完整的物种范围内的泛NLRome在拟南芥序列富集和长读段测序的基础上。泛NLRome在很大程度上饱和约40精心挑选的野生菌株，与泛NLRome的一半是目前在大多数加入。我们图表NLR架构的多样性，确定新的架构，并量化选择性的力量，作用于特定的NLR和NLR域。我们的研究提供了一个蓝图，定义泛NLRomes。
Infectious disease is both a major force of selection in nature and a prime cause of yield loss in agriculture. In plants, disease resistance is often conferred by nucleotide-binding leucine-rich repeat (NLR) proteins, intracellular immune receptors that recognize pathogen proteins and their effects on the host. Consistent with extensive balancing and positive selection, NLRs are encoded by one of the most variable gene families in plants, but the true extent of intraspecific NLR diversity has been unclear. Here, we define a nearly complete species-wide pan-NLRome in Arabidopsis thaliana based on sequence enrichment and long-read sequencing. The pan-NLRome largely saturates with approximately 40 well-chosen wild strains, with half of the pan-NLRome being present in most accessions. We chart NLR architectural diversity, identify new architectures, and quantify selective forces that act on specific NLRs and NLR domains. Our study provides a blueprint for defining pan-NLRomes.