Aquaporin 8 expression is reduced and regulated by microRNAs in patients with ulcerative colitis
Aquaporin 8 expression is reduced and regulated by microRNAs in patients with ulcerative colitis
复制标题
DOI：
10.3760/cma.j.issn.0366-6999.20122989
复制
发表时间：
2013-04-20
影响因子：
6.1
通讯作者：
Yang Yun-sheng
中科院分区：
文献类型：
--
作者：
Min Min;Peng Li-hua;Yang Yun-sheng
背景溃疡性结肠炎（UC）与炎症和组织重塑相关基因的差异表达有关。microRNA（miRNA）通过在转录后水平调控基因表达，控制重要的生理过程，在UC的发病机制中发挥重要作用。本研究的目的是确定水通道蛋白8（AQP 8）的表达及其与miRNA的关系在UC patients.Methods人结肠标本，在这项研究中，从20例UC患者和16名健康受试者在2009年12月至2010年6月在中国人民解放军总医院接受诊断性结肠镜检查。我们使用全基因组微阵列、定量逆转录-聚合酶链反应（qRT-PCR）和蛋白质印迹法从UC组织和健康受试者中筛选不同的基因。通过荧光素酶报告基因构建体分析和特异性miRNA模拟物和抑制剂的转染来评估miRNA对基因表达的调节。结果与健康受试者相比，我们确定了UC患者中1596个基因增加，1301个基因减少。其中，我们重点分析了AQP 8在UC组织中的表达，其在UC组织中的表达减少了3倍（P
Background Ulcerative colitis (UC) is associated with differential expression of genes involved in inflammation and tissue remodeling. MicroRNA (miRNA) plays an important role in the pathogenesis of UC by regulating the gene expression at the post-transcriptional level and control crucial physiological processes. This study aimed to identify aquaporin 8 (AQP8) expression and its relationship with miRNA in UC patients.Methods Human colon samples, in this study, were obtained from 20 patients with UC and 16 healthy subjects undergoing diagnostic colonoscopy at the Chinese People's Liberation Army General Hospital between December 2009 and June 2010. We screened different genes from UC tissues and healthy subjects using genome-wide microarray, quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Western blotting. Regulation of gene expression by miRNAs was assessed by luciferase reporter construct assays and transfection of specific miRNA mimics and inhibitor.Results We identified that 1596 genes were increased and 1301 genes were decreased in UC patients compared to healthy subjects. Among them, we focused on the analysis of AQP8 which was decreased three folds in UC tissues (P