Analysis of the alcABC operon encoding alcaligin biosynthesis enzymes in Bordetella bronchiseptica.
Analysis of the alcABC operon encoding alcaligin biosynthesis enzymes in Bordetella bronchiseptica.
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支气管败血博德特氏菌中编码 alcaligin 生物合成酶的 alcABC 操纵子的分析。
DOI：
10.1016/s0378-1119(97)00094-2
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发表时间：
1997
期刊：
影响因子：
3.5
通讯作者：
Dyer,DW
中科院分区：
文献类型：
--
作者：
Giardina,PC;Foster,LA;Toth,SI;Roe,BA;Dyer,DW
我们之前克隆了一个B。支气管败血症（Bb）基因组DNA片段，补充了Bb alcaligin生物合成突变体，并报告了一个基因，alcA，与预测的蛋白质序列相似性铁载体生物合成酶从其他生物体的鉴定。在本研究中，我们表明，进一步的nt测序这一地区揭示了两个开放的阅读框架（ORF）3'到alcA编码推定的蛋白质AlcB和AlcC，具有显着的序列相似性的好氧肌动蛋白生物合成酶IucB和IucC，分别。RT-PCR分析表明，这三个ORF编码在一个单一的转录本上，并且该操纵子在转录水平上被Fe抑制。引物延伸分析将转录起始点（tsp）置于距离Fur共有序列的5'端35 nt和距离AlcA的推定翻译起始点188 nt处。
We previously cloned a B. bronchiseptica (Bb) genomic DNA fragment that complements a Bb alcaligin biosynthesis mutant, and reported the identification of a gene, alcA, with predicted protein sequence similarity to siderophore biosynthesis enzymes from other organisms. In the present study we show that further nt sequencing of this region revealed two open reading frames (ORFs) 3' to alcA that encode putative proteins AlcB and AlcC, with significant sequence similarity to the aerobactin biosynthesis enzymes IucB and IucC, respectively. RT-PCR analysis indicated that the three ORFs are encoded on a single transcript, and that this operon is repressed at the transcriptional level by Fe. Primer extension analysis placed the transcriptional start point (tsp) 35 nt from the 5' end of the Fur consensus sequence and 188 nt from the putative start of translation of AlcA.