Urine macrophage migration inhibitory factor concentrations as a diagnostic tool in human renal allograft rejection
Urine macrophage migration inhibitory factor concentrations as a diagnostic tool in human renal allograft rejection
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DOI：
10.1097/00007890-200106270-00013
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发表时间：
2001-06-27
期刊：
影响因子：
6.2
通讯作者：
Atkins, RC
中科院分区：
文献类型：
--
作者：
Brown, FG;Nikolic-Paterson, DJ;Atkins, RC
背景。巨噬细胞迁移抑制因子 (MIF) 是一种促炎细胞因子，是巨噬细胞和 T 细胞的有效激活剂。先前的研究表明，在急性同种异体移植肾排斥反应中，局部MIF的产生增加，表明它可能在排斥反应过程中发挥重要作用。确定尿液和血清 MIF 浓度是否：（1）在急性排斥反应中升高，（2）可用作区分急性排斥反应（AR）和环孢菌素肾毒性（CyA 毒性）的无创工具。方法。在一项针对 9 名同种异体肾移植患者（5 名急性排斥反应和 4 名稳定）的前瞻性研究中，在移植后的前 14 天通过 ELISA 连续测量了尿液 MIF 浓度。在一项回顾性研究中，测量了 24 名因急性肾移植功能障碍（11 AR、13 CyA 毒性）而进行活检的患者的尿液和血清中的 MIF 浓度。还在23名稳定肾移植患者和10名正常人中测量了尿液和血清MIF。结果，在正常健康对照的尿液中很容易检测到MIF（106+/-61 pg/mu mol肌酐）。在前瞻性研究中，尿液MIF浓度在移植后第1天大幅增加，随后与血清肌酐平行下降。然而，在活检证实急性排斥反应发生之前，尿液MIF增加。回顾性研究表明，AR患者尿液中MIF浓度比正常对照增加5倍（439+/-313 pg/mu mol Cr；P
Background. Macrophage migration inhibitory factor (MIF) is a pro-inflammatory cytokine that is a potent activator of macrophages and T cells. Previous studies have shown that local MIF production is increased in acute renal allograft rejection, suggesting that it may play an important role in the rejection process,Aims. To determine if urine and serum MIF concentrations: (1) are increased in acute rejection, and (2) can be used as noninvasive tools to discriminate between acute rejection (AR) and cyclosporine nephrotoxicity (CyA toxicity).Methods. In a prospective study of nine renal allograft patients (five acute rejection and four stable), serial urine MIF concentrations were measured by ELISA in the first 14 days after transplantation. In a retrospective study, MIF concentrations in urine and serum were measured in 24 patients who were biopsied for acute renal transplant dysfunction (11 AR, 13 CyA toxicity). Urine and serum MIF were also measured in 23 stable renal transplant patients and 10 normals.Results, MIF was readily detected in the urine of normal healthy controls (106+/-61 pg/mu mol creatinine), In the prospective study, the urinary MIF concentration was increased substantially on day 1 posttransplantation and subsequently fell in parallel with the serum creatinine, However, urine MIF increased before episodes of biopsy proven acute rejection. The retrospective study showed that urine MIF concentrations in patients with AR were increased 5-fold compared to normal controls (439+/-313 pg/mu mol Cr; P