Exercise training decreases DNA damage and increases DNA repair and resistance against oxidative stress of proteins in aged rat skeletal muscle
Exercise training decreases DNA damage and increases DNA repair and resistance against oxidative stress of proteins in aged rat skeletal muscle
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DOI：
10.1007/s00424-002-0918-6
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发表时间：
2002-11-01
影响因子：
4.5
通讯作者：
Goto, S
中科院分区：
文献类型：
--
作者：
Radák, Z;Naito, H;Goto, S
有规律的体育锻炼延缓了一些与年龄相关的疾病，尽管自由基的产生是显着增强与运动的矛盾。八周的跑步机跑步导致中年（20个月大）和老年（30个月大）大鼠的最大摄氧量增加了近40%。腓肠肌中8-羟基-2 '-脱氧鸟苷（8-OHdG）含量的增龄性增加通过运动显著减弱。通过切除P-32标记的受损寡核苷酸测量的8-OHdG修复在运动动物的肌肉中增加。蛋白质的活性羰基衍生物（RCD）并没有随着老化而增加。然而，当肌肉匀浆暴露于1 mM硫酸铁和50 mM抗坏血酸的混合物时，老年对照动物的肌肉比训练组或成年组积累了更多的RCD。老年训练大鼠肌肉中蛋白酶体复合物的胰凝乳蛋白酶样活性增加。我们认为，定期运动诱导的适应减弱了与年龄相关的8-OHdG水平的增加，并增加了DNA修复和抗氧化应激蛋白质的活性。
Regular physical exercise retards a number of age-associated disorders, in spite of the paradox that free radical generation is significantly enhanced with exercise. Eight weeks of treadmill running resulted in nearly a 40% increase in maximal oxygen uptake in both middle-aged (20-month-old) and aged (30-month-old) rats. The age-associated increase in 8-hydroxy-2'-deoxyguanosine (8-OHdG) content was significantly attenuated in gastrocnemius muscle by exercise. The 8-OHdG repair, as measured by the excision of P-32-labeled damaged oligonucleotide, increased in muscle of exercising animals. The reactive carbonyl derivatives (RCD) of proteins did not increase with aging. However, when the muscle homogenate was exposed to a mixture of 1 mM iron sulfate and 50 mM ascorbic acid, the muscle of old control animals accumulated more RCD than that of the trained or adult groups. The chymotrypsin-like activity of proteasome complex increased in muscle of old trained rats. We suggest that regular exercise-induced adaptation attenuates the age-associated increase in 8-OHdG levels, and increases the activity of DNA repair and resistance against oxidative stress in proteins.