Cryo-EM of bacterial pili and archaeal flagellar filaments
Cryo-EM of bacterial pili and archaeal flagellar filaments
复制标题
DOI：
10.1016/j.sbi.2017.05.012
复制
发表时间：
2017-10-01
影响因子：
6.8
通讯作者：
Egelman, Edward H.
中科院分区：
文献类型：
--
作者：
Egelman, Edward H.
低温电子显微镜（cryo-EM）的最新进展已经开启了一种可能性，即一大类生物结构，螺旋聚合物，现在可以很容易地在近原子分辨率下重建。这将产生巨大的影响，因为这些结构中的大多数不太可能结晶。本文综述了低温电子显微镜对三种类型细菌（伴侣引导、交配和IV型）以及古细菌鞭毛细丝的研究进展。虽然人们早就知道古细菌鞭毛细丝内的一个结构域与细菌IV型柱内的一个结构域同源，但新的研究揭示了同源甚至高度保守的亚基如何以不同的方式包装在一起，而序列上只有很小的变化。
Recent advances in cryo-electron microscopy (cryo-EM) have opened up the possibility that a large class of biological structures, helical polymers, may now be readily reconstructed at near-atomic resolution. This will have a huge impact, since most of these structures are unlikely to be crystallized. This review focuses on new cryo-EM studies involving three classes of bacterial pill (chaperone-usher, mating, and Type IV) as well as on archaeal flagellar filaments. While it has long been known that one domain within archaeal flagellar filaments is homologous to a domain within bacterial Type IV pilins, the new studies shed light on how homologous and even highly conserved subunits can pack together in different ways with only small changes in sequence.