RIP to the PIP: PCNA-binding motif no longer considered specific: PIP motifs and other related sequences are not distinct entities and can bind multiple proteins involved in genome maintenance
RIP to the PIP: PCNA-binding motif no longer considered specific: PIP motifs and other related sequences are not distinct entities and can bind multiple proteins involved in genome maintenance
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DOI：
10.1002/bies.201600116
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发表时间：
2016-11-01
期刊：
影响因子：
4
通讯作者：
Washington, M. Todd
中科院分区：
文献类型：
--
作者：
Boehm, Elizabeth M.;Washington, M. Todd
许多负责基因组维护的蛋白质通过短序列基序相互作用。其中最著名的是PIP基序，它介导与复制蛋白PCNA的相互作用。其他包括RIR基序，其结合跨损伤合成蛋白Rev 1，和MIP基序，其结合错配修复蛋白Mlh 1。虽然这些基序具有相似的共有序列，但它们传统上被视为单独的基序，每个基序都有自己的靶蛋白。在这篇文章中，我们回顾了最近几项挑战这一观点的研究。综合起来，它们意味着这些不同的图案不是不同的实体。相反，有一个单一的，更广泛的一类基序，我们称之为PIP样基序，具有重叠的特异性，能够结合多种靶蛋白。鉴于此，我们必须重新评估这些基序在形成负责基因组维护的相互作用蛋白质网络中的作用。
Many proteins responsible for genome maintenance interact with one another via short sequence motifs. The best known of these are PIP motifs, which mediate interactions with the replication protein PCNA. Others include RIR motifs, which bind the translesion synthesis protein Rev1, and MIP motifs, which bind the mismatch repair protein Mlh1. Although these motifs have similar consensus sequences, they have traditionally been viewed as separate motifs, each with their own target protein. In this article, we review several recent studies that challenge this view. Taken together, they imply that these different motifs are not distinct entities. Instead, there is a single, broader class of motifs, which we call PIP-like motifs, which have overlapping specificities and are capable of binding multiple target proteins. Given this, we must reassess the role of these motifs in forming the network of interacting proteins responsible for genome maintenance.