A new class of nitrobenzoic acid-based AIE photosensitizers for highly efficient photodynamic antibacterial therapy
A new class of nitrobenzoic acid-based AIE photosensitizers for highly efficient photodynamic antibacterial therapy
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DOI：
10.1007/s40843-021-1652-2
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发表时间：
2021-05
期刊：
影响因子：
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通讯作者：
Huanhuan Wang;X. Pan;Yaqi Wang;Wenzhen Liu;T. Dai;Binbin Yuan;Xueyuan Chen;Zhuo Chen
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作者：
Huanhuan Wang;X. Pan;Yaqi Wang;Wenzhen Liu;T. Dai;Binbin Yuan;Xueyuan Chen;Zhuo Chen
光动力疗法（PDT）在抗菌领域越来越受到关注。传统的光敏剂（PS）容易在水介质中聚集，从而减少活性氧（ROS）的产生并严重影响光动力功效。人们已经做出了许多努力来防止传统 PS 的聚集。相比之下，聚集诱导发射 PS (AIE-PS) 利用聚集来提高 ROS 生成和荧光强度。然而，已报道的抗菌AIE-PSs的功效较差。在此，我们报道了一类基于硝基苯甲酸结构的新型高效抗菌AIE-PS。 TTVBA是一种带负电荷的AIE-PS，不仅可以选择性杀灭球形细菌（金黄色葡萄球菌（S. aureus））而不是杆状细菌（大肠杆菌（E. coli）），而且可以很容易地扩展到多种带正电荷的AIE-PS（TTVBP1-3），具有广谱抗菌活性。我们证明 TTVBP2 可以杀死 3.0 log10ofS。在极低浓度（125 nmol L−1）的金黄色葡萄球菌下，TTVBP3在1 µmol L−1浓度下可杀死4.7 log10的表皮葡萄球菌（S. epidermidis）和3.8 log10的E。 coliat 5 µmol L−1，从而使它们成为迄今为止报道的最有效的抗菌 AIE-PS 之一。同时，这些AIE-PS通过与细菌简单混合，表现出优异的细菌免洗成像能力。因此，我们设想 TTVBA，一种基于硝基苯甲酸的可扩展 AIE PS，为抗菌治疗中 AIE-PS 的设计提供了一条新途径。
Photodynamic therapy (PDT) has been drawing more and more attention in the antibacterial field. Traditional photosensitizers (PSs) tend to aggregate in aqueous media, which reduces the generation of reactive oxygen species (ROS) and seriously affects the photodynamic efficacy. Many efforts have been made to prevent aggregation of traditional PSs. By contrast, aggregation-induced emission PSs (AIE-PSs) take advantage of aggregation to boost ROS generation and fluorescence intensity. However, the efficacies of the reported antibacterial AIE-PSs are poor. Herein, we report a new class of highly effective antibacterial AIE-PSs based on nitrobenzoic acid structure. TTVBA, a negatively charged AIE-PS, can not only selectively kill spherical bacteria (Staphylococcus aureus(S. aureus)) rather than rod-shaped bacteria (Escherichia coli(E. coli)), but also be easily extended to several AIE-PSs (TTVBP1-3) with positive charges and broad-spectrum antibacterial activity. We demonstrate that TTVBP2 can kill 3.0 log10ofS. aureusat very low concentration (125 nmol L−1), TTVBP3 can kill 4.7 log10ofStaphylococcus epidermidis(S. epidermidis) at a concentration of 1 µmol L−1and 3.8 log10ofE. coliat 5 µmol L−1, thus enabling them among the most effective antibacterial AIE-PSs reported so far. Meanwhile, these AIE-PSs exhibit excellent wash-free imaging ability for bacteria by simple mixing with bacteria. We thus envision that TTVBA, a nitrobenzoic acid-based extendable AIE PS, provides a new route for the design of AIE-PSs in antibacterial treatment.