Scribble and Discs-large direct initial assembly and positioning of adherens junctions during the establishment of apical-basal polarity
Scribble and Discs-large direct initial assembly and positioning of adherens junctions during the establishment of apical-basal polarity
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DOI：
10.1242/dev.180976
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发表时间：
2019-11-15
期刊：
影响因子：
4.6
通讯作者：
Peifer, Mark
中科院分区：
文献类型：
--
作者：
Bonello, Teresa T.;Choi, Wangsun;Peifer, Mark
顶基极性是动物组织的一个基本特性。果蝇胚胎为定义启动和维持极性的机制提供了一个杰出的模型。极性是在细胞化过程中开始的，当细胞-细胞粘附体连接位于顶端和基底外侧结构域的未来边界时。极性维持涉及到顶端和基部极性复合物之间的互补和拮抗相互作用。Scribble/Dlg模块以在极性维护期间促进基底侧身份而闻名。在这里，我们报告了Scribble/Dlg在极性起始中的一个令人惊讶的作用，将其放置在网络定位粘附连接的顶部附近。Scribble和Dlg富集于粘附体初生连接处，是粘附体连接处定位和超分子组装所必需的，也在粘附体基部连接处组装中起作用。我们测试了潜在机制的假设，探索了对蛋白质运输、细胞骨架极性或Par-1定位/功能的潜在影响。我们的数据表明Scribble/Dlg模块在极性起始中起多重作用。Scribble的不同领域促成了这些不同的角色。总之，这些数据揭示了Scribble/Dlg作为主支架在不同时间和地点调节不同连接复合物组装的新作用。
Apical-basal polarity is a fundamental property of animal tissues. Drosophila embryos provide an outstanding model for defining mechanisms that initiate and maintain polarity. Polarity is initiated during cellularization, when cell-cell adherens junctions are positioned at the future boundary of apical and basolateral domains. Polarity maintenance then involves complementary and antagonistic interplay between apical and basal polarity complexes. The Scribble/Dlg module is well-known for promoting basolateral identity during polarity maintenance. Here, we report a surprising role for Scribble/Dlg in polarity initiation, placing it near the top of the network-positioning adherens junctions. Scribble and Dlg are enriched in nascent adherens junctions, are essential for adherens junction positioning and supermolecular assembly, and also play a role in basal junction assembly. We test the hypotheses for the underlying mechanisms, exploring potential effects on protein trafficking, cytoskeletal polarity or Par-1 localization/function. Our data suggest that the Scribble/Dlg module plays multiple roles in polarity initiation. Different domains of Scribble contribute to these distinct roles. Together, these data reveal novel roles for Scribble/Dlg as master scaffolds regulating assembly of distinct junctional complexes at different times and places.