Chemical, physicochemical and spectrophotometric properties of crystalline chlorophyll-protein complexes from Lepidium virginicum L.
Chemical, physicochemical and spectrophotometric properties of crystalline chlorophyll-protein complexes from Lepidium virginicum L.
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独行菜结晶叶绿素-蛋白质复合物的化学、物理化学和分光光度特性。
DOI：
10.1016/0005-2728(81)90032-3
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
C. Ishikawa
中科院分区：
文献类型：
--
作者：
T. Murata;C. Ishikawa
用硫酸铵分级，DEAE-纤维素柱层析和Sephacryl S-200柱层析，从大棚里采集的加州莫诺湖附近的种子和日本千叶奈里野采集的种子中，制备了两种水溶性叶绿素-蛋白质复合体，其中一种来自温室栽培的植物(CP661)，另一种来自日本千叶奈良野的植物。通过结晶进一步纯化了叶绿素·蛋白。CP661在661、468、439、419、380、339和272 nm处有吸收峰。CP663在663、469、438、419、379、338和272 nm处有吸收峰。凝胶过滤层析法测得CP661和CP663的相对分子质量分别为78000和80000；平衡沉淀法测得CP661和CP663的相对分子质量分别为83000和107000。1摩尔的叶绿素·蛋白中含有4摩尔的叶绿素，其比值在CP661中为1.0，在CP663中为1.6~1.9，但不含类胡萝卜素。这些特性不同于由含有洗涤剂的叶绿体类囊体膜制备的叶绿素-蛋白质复合体。
Two kinds of water-soluble chlorophyll-protein complexes were prepared from leaves ofLepidium virginicumL., one (CP661) from the plant cultivated in a green house from seeds collected near Mono Lake, CA, and the other (CP663) from a plant collected at Narashino, Chiba, Japan, by ammonium sulfate fractionation followed by column chromatography on DEAE-cellulose and Sephacryl S-200. The chlorophyll · proteins were further purified by crystallization. CP661 has absorption peaks at 661, 468, 439, 419, 380, 339 and 272 nm. CP663 had absorption peaks at 663, 469, 438, 419, 379, 338 and 272 nm. Estimated molecular weights were 78 000 for CP661 and 80 000 for CP663 by gel filtration chromatography and 83 000 for CP661 and 107 000 for CP663 by an equilibrium sedimentation method. 1 mol chlorophyll · protein contained 4 mol chlorophyllaandbwith ratios of 1.0 in CP661 and 1.6 to 1.9 in CP663, but no carotenoids. These characters are different from those of chlorophyll-protein complexes which are prepared from the thylakoid membranes of chloroplasts with detergents.