Optical mapping of release properties in synapses
Optical mapping of release properties in synapses
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DOI：
10.3389/fncir.2010.00018
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发表时间：
2010-08-12
影响因子：
3.5
通讯作者：
Ryan, Timothy A.
中科院分区：
文献类型：
--
作者：
Ariel, Pablo;Ryan, Timothy A.
突触是决定信息如何流经大脑的重要功能单位。了解它们的生物物理特性和支撑它们的分子是细胞神经科学的一个重要目标。因此，开发能够在允许分子操作的模型系统中轻松测量突触参数的协议是很有意义的。在这里，我们使用了一种灵敏的高时间分辨率光学方法，使我们能够表征对突触前功效至关重要的两个功能参数：囊泡融合概率（Pv）和易释放池大小（RRP）。我们采用了两种不同的方法来确定广泛一致的 RRP 大小：高频刺激消耗 RRP 和单次动作电位刺激期间钙传感器饱和。我们的方法基于提供稳健、定量、纯突触前读数的报告基因，并为研究影响突触小泡胞吐作用的分子提供了新途径。
Synapses are important functional units that determine how information flows through the brain. Understanding their biophysical properties and the molecules that underpin them is an important goal of cellular neuroscience. Thus, it is of interest to develop protocols that allow easy measurement of synaptic parameters in model systems that permit molecular manipulations. Here, we used a sensitive and high-time resolution optical approach that allowed us to characterize two functional parameters critical to presynaptic efficacy: vesicle fusion probability (Pv) and readily-releasable pool size (RRP). We implemented two different approaches to determine the RRP size that were in broad agreement: depletion of the RRP by high-frequency stimulation and saturation of the calcium sensor during single action potential stimuli. Our methods are based on reporters that provide a robust, quantitative, purely presynaptic readout and present a new avenue to study molecules that affect synaptic vesicle exocytosis.