Chemical structure of the galactomannan from the cell wall of Aspergillus niger.
Chemical structure of the galactomannan from the cell wall of Aspergillus niger.
复制标题
黑曲霉细胞壁中半乳甘露聚糖的化学结构。
DOI：
10.1016/s0021-9258(17)40498-4
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发表时间：
1977
期刊：
影响因子：
--
通讯作者：
J. H. Nordin
中科院分区：
文献类型：
--
作者：
P. Bardalaye;J. H. Nordin
对表面生长的黑曲霉菌丝壁进行碱性提取，分离出半乳甘露聚糖，并进行了结构表征。从Bio-Gel P-150柱上的洗脱剖面显示了广泛的分子大小，分为两个部分。气相色谱和比色分析表明，每个馏分由大约等摩尔量的半乳糖和甘露糖加上12%至14%的葡萄糖组成。两者都具有相似的低旋光性，并且含有不稳定的半乳糖。甲基化、Smith降解、乙酰解、与刀豆蛋白A和β - d -半乳糖呋喃苷酶的反应性，以及用d -半乳糖氧化酶消化半乳糖，这些技术被用来确定多糖的共价结构。这些研究结果表明，它由一系列长度为5至9个己糖单位的链组成，由α 1导联在甘露吡喃基之间形成6个键。每条链的外部部分由半乳糖三糖或四糖组成，一般结构为galfbeta1通向4 Galp(1-2) 1通向4 Galp1通向。该片段通过（1 - 2）键连接到内部部分，该部分是甘露吡喃基单位的二糖到五糖，在α - 1 - 2糖苷键中连接。结合轻度酸水解和甲基化实验表明，半乳糖呋喃基末端单元仅附着在半乳糖上。葡萄糖在聚合物整体结构中的组织尚未确定。讨论了该多糖与真菌和酵母半乳甘露聚糖的结构关系。
The galactomannan of surface grown Aspergillus niger has been isolated by alkaline extraction of hyphal walls and characterized structurally. Its elution profile, from a column of Bio-Gel P-150, reveals a broad range of molecular sizes grouped into two fractions. Gas chromatographic and colorimetric analyses indicate that each fraction is composed of approximately equimolar quantities of galactose and mannose plus 12 to 14% glucose. Both have similar low optical rotations and contain acid labile galactose. Methylation, Smith degradation, acetolysis, reactivity with concanavalin A and beta-D-galactofuranosidase, plus digestionof galactose with D-galactose oxidase, were techniques employed to determine the polysaccharide's covalent structure. Results of these studies indicate that it is composed of a series of chains, 5 to 9 hexose units in length, connected by alpha1 leads to 6 bonds between mannopyranosyl moieties. The external portion of each chain consists of a galactose tri- or tetrasaccharide of the general structure Galf beta1 leads to 4 Galp(1-2) 1 leads to 4 Galp1 leads to. This segment is connected via a (1 leads to 2) linkage to the internal portion which is a di- to pentasaccharide of mannopyranosyl units joined in alpha1 leads to 2 glycoside linkage. Combination mild acid hydrolysis and methylation experiments indicate galactofuranosyl terminal units are attached only to galactose. The organization of glucose into the overall structure of the polymer has not been determined. Structural relationships of this polysaccharide to both fungal and yeast galactomannans are discussed.