Glial scar and neuroregeneration: histological, functional, and magnetic resonance imaging analysis in chronic spinal cord injury Laboratory investigation
Glial scar and neuroregeneration: histological, functional, and magnetic resonance imaging analysis in chronic spinal cord injury Laboratory investigation
复制标题
胶质疤痕和神经再生：慢性脊髓损伤的组织学、功能和磁共振成像分析实验室研究
DOI：
10.3171/2010.3.spine09190
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发表时间：
2010-08-01
影响因子：
2.8
通讯作者：
Feng, Hua
中科院分区：
文献类型：
--
作者：
Hu, Rong;Zhou, Jianjun;Feng, Hua
物体。神经胶质瘢痕被认为是脊髓损伤(SCI)后神经再生停止的原因。然而，很少有定量证据表明神经胶质瘢痕与损伤后轴突再生长的关系。在大鼠和狗身上进行的研究中，使用重物坠落伤装置建立了创伤性脊髓损伤模型，并对组织切片进行H&E染色进行免疫组织化学分析。分别采用电生理记录法和Basso-Beattie-Bresnhan运动功能评定量表对模型动物进行功能和行为测试。应用磁共振成像技术对犬脊髓损伤后脊髓空洞进行观察。形态学结果显示，脊髓损伤后4周已形成星形胶质细胞瘢痕。当再生轴突到达病变部位附近时，胶质瘢痕阻碍了再生轴突的延伸。与这些发现一致的是，电生理、行为和活体磁共振成像测试显示，脊髓损伤后4周，功能恢复达到平台期。并测量损伤大鼠脊髓内胶质瘢痕厚度。吻侧和尾侧神经胶质瘢痕到病变腔的平均厚度分别为107.00±20.12mm2和69.92mm2/-15.12mm2。这些结果提供了全面的证据表明，在脊髓损伤后4周，胶质瘢痕的形成抑制了轴突再生。本研究揭示了损伤后恢复的关键时间窗和胶质瘢痕的详细空间定位，为制定胶质瘢痕消融治疗策略提供重要依据。(DOI：10.3171/2010.3.SPINE09190)
Object. A glial scar is thought to be responsible for halting neuroregeneration following spinal cord injury (SCI). However, little quantitative evidence has been provided to show the relationship of a glial scar and axonal regrowth after injury.Methods. In this study performed in rats and dogs, a traumatic SCI model was made using a weight-drop injury device, and tissue sections were stained with H & E for immunohistochemical analysis. The function and behavior of model animals were tested using electrophysiological recording and the Basso-Beattie-Bresnahan Locomotor Rating Scale, respectively. The cavity in the spinal cord after SCI in dogs was observed using MR imaging.Results. The morphological results showed that the formation of an astroglial scar was defined at 4 weeks after SCI. While regenerative axons reached the vicinity of the lesion site, the glial scar blocked the extension of regrown axons. In agreement with these findings, the electrophysiological, behavioral, and in vivo MR imaging tests showed that functional recovery reached a plateau at 4 weeks after SCI. The thickness of the glial scars in the injured rat spinal cords was also measured. The mean thickness of the glial scar rostral and caudal to the lesion cavity was 107.00 +/- 20.12 mu m; laterally it was 69.92 +/- 15.12 mu m.Conclusions. These results provide comprehensive evidence indicating that the formation of a glial scar inhibits axonal regeneration at 4 weeks after SCI. This study reveals a critical time window of postinjury recovery and a detailed spatial orientation of glial scar, which would provide an important basis for the development of therapeutic strategy for glial scar ablation. (DOI: 10.3171/2010.3.SPINE09190)