Anti-sera raised in rabbits against crotoxin and phospholipase A2 from Crotalus durissus cascavella venom neutralize the neurotoxicity of the venom and crotoxin
Anti-sera raised in rabbits against crotoxin and phospholipase A2 from Crotalus durissus cascavella venom neutralize the neurotoxicity of the venom and crotoxin
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DOI：
10.1016/j.toxicon.2004.05.011
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发表时间：
2004-08-01
期刊：
影响因子：
2.8
通讯作者：
Marangoni, S
中科院分区：
文献类型：
--
作者：
Beghini, DG;Hernandez-Oliveira, S;Marangoni, S
鳄鱼毒素是南美响尾蛇和硬壳响尾蛇毒液中的主要神经毒素，含有碱性磷脂酶A(2)和酸性蛋白克罗波汀。在本工作中，我们观察了兔抗巴豆腐毒素及其磷脂酶A(2)亚单位的抗血清对小鼠膈神经-横隔膜和鸡颈二腔肠系膜细胞的毒性作用。免疫印迹实验表明，该抗血清可识别卡氏梭菌毒素和聚乳酸(2)。两种抗血清的终点稀释度均为3×10(-6)，经酶联免疫吸附试验证实。在1：2和1：3的毒液和抗血清比例下，抗巴毒素血清可中和膈神经-膈肌制剂的神经肌肉阻断作用。抗解放军(2)血清在毒液或巴豆毒素与抗血清比例为1：1时也可中和这种神经肌肉活性。在颈二头虫制剂中，抗毒素血清的相应比例为1：3，抗解放军(2)血清的相应比例为1：1和1：2。该抗血清在小鼠制剂中的中和能力与市售的恐怖钩端螺旋体抗血清相当。结果表明，鹿茸蛇毒抗血清和磷脂酶A(2)抗血清可中和蛇毒和巴豆毒素对神经肌肉制剂的神经肌肉阻断作用，但抗巴毒素抗血清的效力略弱于巴豆毒素抗血清。(C)2004爱思唯尔有限公司。保留所有权利。
Crotoxin, the principal neurotoxin in venom of the South American rattlesnakes Crotalus durissus terrificus and Crotalus durissus cascavella, contains a basic phospholipase A(2) (PLA(2)) and an acidic protein, crotapotin. In this work, we examined the ability of rabbit anti-sera against crotoxin and its PLA(2) subunit to neutralize the neurotoxicity of venom and crotoxin from C. d. cascavella in mouse phrenic nerve-diaphragm and chick biventer cervicis preparations. Immunoblotting showed that the anti-sera recognized C. d. cascavella crotoxin and PLA(2). This was confirmed by ELISA, with both anti-sera having end-point dilutions of 3 x 10(-6). Anti-crotoxin serum neutralized the neuromuscular blockade in phrenic nerve-diaphragm muscle preparations at venom or crotoxin:anti-serum ratios of 1:2 and 1:3, respectively. Anti-PLA(2) serum also neutralized this neuromuscular activity at a venom or crotoxin:anti-serum ratio of 1:1. In biventer cervicis preparations, the corresponding ratio for anti-crotoxin serum was 1:3 for venom and crotoxin, and 1:1 and 1:2 for anti-PLA(2) serum. The neutralizing capacity of the sera in mouse preparations was comparable to that of commercial anti-serum raised against C. d. terrificus venom. These results show that anti-sera against crotoxin and PLA(2) from C. d. cascavella venom neutralized the neuromuscular blockade induced by venom and crotoxin in both nerve-muscle preparations, with the anti-serum against crotoxin being slightly less potent than that against crotoxin. (C) 2004 Elsevier Ltd. All rights reserved.