Significant Increase of Prostaglandin E-Major Urinary Metabolite in Male Smokers: A Screening Study of Age and Gender Differences Using a Simple Radioimmunoassay
Significant Increase of Prostaglandin E-Major Urinary Metabolite in Male Smokers: A Screening Study of Age and Gender Differences Using a Simple Radioimmunoassay
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DOI：
10.1002/jcla.21640
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发表时间：
2014-01-01
影响因子：
2.7
通讯作者：
Fujiwara, Mutsunori
中科院分区：
文献类型：
--
作者：
Okayasu, Isao;Ohnishi, Hiromitsu;Fujiwara, Mutsunori
背景为了评估炎症状态，我们开发了一种简单、可靠的前列腺素 E-主要尿代谢物 (PGE-MUM) 放射免疫测定法 (RIA)，该代谢物在代谢后在尿液中保持稳定。利用该方法，我们进行了一项筛选研究，比较健康检查志愿者的PGE-MUM与血清C反应蛋白（CRP）水平的标准值。方法使用新开发的RIA对797例健康检查志愿者的正常尿液样本进行PGE-MUM（微克/克肌酐）测量，并分析其与年龄、性别、吸烟和饮酒习惯的关系。将结果与血清CRP进行比较。结果男性中PGE-MUM显着高于女性。与从不吸烟的男性（非吸烟者）相比，吸烟男性的这一比例明显更高，尤其是 40 岁以上的男性。在非吸烟者中，男性 PGE-MUM 随着年龄的增长而下降，而女性则上升。虽然PGE-MUM反映了当前的吸烟状况，与吸烟指数(SI)无关，但血清CRP可以指示当前和以前的吸烟状况，而与SI有关。结论吸烟者中的PGE-MUM增加，正如肺组织可能的炎症损伤所表明的那样。因此，与血清 CRP 不同，这种 PGE-MUM 的 RIA 方法可能是当前炎症的敏感且可靠的生物标志物。 (C) 2013 年 Wiley 期刊公司。
BackgroundFor the assessment of inflammatory status, we have developed a simple, reliable radioimmunoassay (RIA) of prostaglandin E-major urinary metabolite (PGE-MUM), which remains stable in urine after it is metabolized. Using this method, we conducted a screening study to compare standard values of PGE-MUM to serum C-reactive protein (CRP) levels in health check volunteers.MethodsPGE-MUM (micrograms per gram creatinine) was measured in normal urine samples obtained from 797 samples in volunteers for health check, using a newly developed RIA, and analyzed in relation to age, gender, smoking, and drinking habits. Results were compared to serum CRP.ResultsPGE-MUM was significantly higher in males than in females. It was significantly higher in smoking males, compared to males who had never smoked (nonsmokers), particularly in those above 40 years of age. In nonsmokers, PGE-MUM declined in males with advancing age, while it rose in females. Although PGE-MUM reflected current smoking status, independent of smoking index (SI), serum CRP indicated both current and former smoking condition, rather dependent upon SI.ConclusionsPGE-MUM increases in smokers, as suggested by possible inflammatory injury of pulmonary tissue. This RIA method for PGE-MUM may be thus a sensitive and reliable biomarker for current inflammation, different from serum CRP. (C) 2013 Wiley Periodicals, Inc.